pLOC-MCOLN1
(Plasmid #131583)

• Purpose: For expression of human MCOLN1 in mammalian cells, GFP-tagged
• Depositing Lab: Sean Morrison
• Publication: Kasitinon et al Cell Rep. 2019 Aug 27;28(9):2293-2305.e9. doi: 10.1016/j.celrep.2019.07.086.

Backbone

• Vector backbone: pLOC
• Backbone manufacturer: Dharmacon
• Backbone size w/o insert (bp): 9089
• Total vector size (bp): 10832
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MCOLN1
• Alt name: TRPML1, Mucolipin-1
• Species: H. sapiens (human)
• Insert Size (bp): 1743
• Entrez Gene: MCOLN1 (a.k.a. MG-2, ML1, ML4, MLIV, MST080, MSTP080, TRP-ML1, TRPM-L1, TRPML1)
• Promoter: CMV
• Tag / Fusion Protein:
  • turboGFP tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The ORF was from DNASU

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry