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Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTARA
Plasmid#31491DepositorInsertRNA polymerase
UseTagsExpressionBacterialMutationN823DPromoterAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_18
Plasmid#59953PurposeQuad split (at 61, 179, 601-SZ)DepositorInsertFour-fragment T7* RNA polymerase
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_14
Plasmid#59952PurposeTriple split (at 67, 601-SZ)DepositorInsertThree-fragment T7* RNA polymerase
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMABC
Plasmid#45398DepositorUseTagsExpressionBacterialMutationPromoterNone and T7Available sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAB50
Plasmid#101678PurposemCherry under T7 promoter controlDepositorInsertmCherry under T7 promoter control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHSSd_41
Plasmid#59964PurposeHigh full length T7 RNAP* expressionDepositorInsertT7* RNAP
UseTagsExpressionBacterialMutationN/APromoterPJ23105Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAB143
Plasmid#101679PurposeLAA-tagged mCherry under T7 promoter controlDepositorInsertLAA-tagged mCherry under T7 promoter control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-CGG-R12-KIR
Plasmid#63626PurposeCGG-R12-KIR RNAP mutantDepositorInsertK1F IKR RNAP
UseTagsExpressionMutationQ744K, L747V, N748H, L749I, R756E, L757M, H772RPromoterT5/LacAvailable sinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-CGG-R12-KIRV
Plasmid#63668PurposeCGG-R12-KIRV RNAP mutantDepositorInsertCGG-R12-KIRV RNAP
UseTagsExpressionMutationQ744K, L747V, N748H, L749I, R756E, L757M, H772R, …PromoterT5/LacAvailable sinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAB213
Plasmid#101677PurposemCherry under T7 promoter controlDepositorInsertmCherry under T7 promoter control
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-T3-R5-RV
Plasmid#63628PurposeT3-R5-RV RNAP mutantDepositorInsertT3-R5-RV RNAP
UseTagsExpressionMutationT745K, N748D, L749M, M750I, H772R, E775VPromoterT5/LacAvailable sinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-T3-R5-RRVH
Plasmid#63623PurposeT3-R5-RRVH RNAP mutantDepositorInsertT3 RRVH RNAP
UseTagsExpressionMutationT745K, N748D, L749M, M750I, G753R, H772R, E775V, …PromoterT5/LacAvailable sinceJuly 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-KIF-R5-IRH
Plasmid#63629PurposeK1F-R5-IRH RNAP mutantDepositorInsertKIF-R5-IRH RNAP
UseTagsExpressionMutationL749I, Q754S, R756N, I761V, H772R, and Q786HPromoterT5/LacAvailable sinceJune 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE-WT-KIF-R5-KIKR
Plasmid#63624PurposeKIF-R5-KIKR RNAP mutantDepositorInsertKIF-R5-KIKR RNAP
UseTagsExpressionMutationQ744K, L749I, M750K, Q754S, R756N, I761V, H772RPromoterT5/LacAvailable sinceApril 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTHSSd_23
Plasmid#59954PurposePTac T7 sigma fragment expressionDepositorInsertT7 sigma fragment
UseTagsExpressionBacterialMutationN/APromoterPTacAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiTagsExpressionMutationPromoteropposing T7 promotersAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-WT-CTGA-R13-AKSIRV
Plasmid#63627PurposeCTGA-R13-AKSIRV RNAP mutantDepositorInsertCTGA-R13-AKSIRV RNAP
UseTagsn/aExpressionMutationV725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …PromoterT5/LacAvailable sinceJune 3, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits