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Plasmid#225788PurposeRS421 library variant 13168DepositorInsert13168_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_89
Plasmid#225803PurposeRS421 library variant 13168DepositorInsert13168_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEP1
Plasmid#61046PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a_UDP_Xase
Plasmid#73158PurposeExpresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669DepositorInsertpET24a
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLU_101
Plasmid#225814PurposeRS421 library variant 14434DepositorInsert14434_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLZts
Plasmid#128799PurposeTemperature-sensitive vector for allelic replacement mutagenesis of Group A StreptococcusDepositorTypeEmpty backboneUseBacterial mutagenesisTagsExpressionMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLU_83
Plasmid#225784PurposeRS421 library variant 14434 in IDT backboneDepositorInsert14434_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-Plac
Plasmid#122632PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-Plac
Plasmid#122634PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEPX
Plasmid#122631PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPhygCAS9
Plasmid#63555PurposeExpresses CAS9 in LeishmaniaDepositorInsertHumanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
UseLeishmania expressionTagsflagExpressionMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBFusion1
Plasmid#133438PurposeStreptococcus GB1 domain in vector for bacterial expression.DepositorInsertGB1 domain
UseTagsExpressionBacterialMutationThis fragment encodes only aa 1 to 56.PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a_UDP_Xase_E113
Plasmid#73159PurposeExpresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E113ADepositorInsertpET24a
UseTagsExpressionBacterialMutationchange glutamate 113 to alaninePromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a_UDP_Xase_E162
Plasmid#73160PurposeExpresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162ADepositorInsertpET24a
UseTagsExpressionBacterialMutationchange glutamate 162 to alaninePromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpIMPDH
Plasmid#206980PurposeStreptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.DepositorInsertSpIMPDH (guaB Streptococcus pyogenes)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScCas9
Plasmid#117700PurposeMammalian expression vector encoding WT ScCas9DepositorInsertStreptococcus canis Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPEPZ-Plac
Plasmid#122635PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only