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Plasmid#130230PurposeEMMA Kit # 1000000119 extensionDepositorInsertSirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI
Plasmid#130197PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-021 Lamp1-Sirius//BsaI
Plasmid#130202PurposeEMMA Kit # 1000000119 extensionDepositorInsertLamp1-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-011 Vimentin-Sirius//BsaI
Plasmid#130192PurposeEMMA Kit # 1000000119 extensionDepositorInsertVimentin-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-006 EB3-Sirius//BsaI
Plasmid#130187PurposeEMMA Kit # 1000000119 extensionDepositorInsertEB3-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-001 LifeAct-Sirius//BsaI
Plasmid#130182PurposeEMMA Kit # 1000000119 extensionDepositorInsertLifeAct-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSKΔBS-SD_MCS_SA(NBSI)-24xMS2-sirius-96xopto-TetO-Int-3xFLAG-NeoR-GS4
Plasmid#177265PurposeSTREAMING-tag cassetteDepositorInsertSTREAMING-tag cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-HALOnls
Plasmid#121937PurposeCRISPR-Sirius plasmidDepositorInsertMCP-HALOnls
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianMutationPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNK141a2
Plasmid#156374PurposeExpresses orthogonal RBS Sirius reporterDepositorInsertSirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBD21_033
Plasmid#109150PurposepL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of SiriusDepositorInsertsmRFP
Sirius
UseSynthetic BiologyTagsExpressionBacterial and MammalianMutationPromoterCMV and pTetAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP
Plasmid#185013PurposeBacterial Expression of 6xHis Sirius-GFP Fusion ProteinDepositorInsertSirius-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Sec61β
Plasmid#174466PurposeExpress EGFP with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertSirius with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-NFYC-IDR-mEGFP
Plasmid#185015PurposeBacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion ProteinDepositorInsertNFYC IDR (Nfyc Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG S297A
Plasmid#169992PurposeMammalian purification construct for CARD8 with an autoprocessing-deficient mutationDepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-siriusGFP-FLAGExpressionMammalianMutationS297APromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only