-
Plasmid#48118PurposeShuttle vector E. coli/B.meg.; encoding the signal peptide for protein secretion of the Bacillus megaterium protein YngK under control of the xylose inducible promoterDepositorInsertsignal peptide of YngK
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
-
pTn7xTS_SpyCatcher:mNG
Plasmid#225185PurposeUsed for inserting SpyCatcher fused to mNeonGreen at the bacterial Tn7 siteDepositorInsertSpyCatcher:mNeonGreen
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher:AausFP1
Plasmid#225186PurposeUsed for inserting SpyCatcher fused to AausFP1 at the bacterial Tn7 siteDepositorInsertSpyCatcher:AausFP1
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher:mCitrine
Plasmid#225187PurposeUsed for inserting SpyCatcher fused to mCitrine at the bacterial Tn7 siteDepositorInsertSpyCatcher:mCitrine
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher:mKate2
Plasmid#225188PurposeUsed for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 siteDepositorInsertSpyCatcher:mKate2
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher:mTFP1
Plasmid#225189PurposeUsed for inserting SpyCatcher fused to mTFP1 at the bacterial Tn7 siteDepositorInsertSpyCatcher:mTFP1
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SpyTag
Plasmid#225193PurposeUsed for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SpyTag003
Plasmid#225194PurposeUsed for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag003
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpy
Plasmid#220184PurposesgRNA expression vector - promoterless backboneDepositorInsertpromoterless backbone
UseCRISPRTagsExpressionBacterialMutationPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA
Plasmid#220185PurposesgRNA expression vector - pEM7 promoterDepositorInsertpEM7 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyC
Plasmid#220187PurposesgRNA expression vector - pBG42 promoterDepositorInsertpBG42 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD
Plasmid#220188PurposesgRNA expression vector - pBG28 promoterDepositorInsertpBG28 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pspy_STD
Plasmid#165806Purposebacterial terminatorDepositorInsertspy
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-mi3
Plasmid#159995PurposeExpresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccinationDepositorInsertSpyCatcher003-mi3
UseTagsC-TagExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
UseTagsGSGSGS linker and His6ExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
UseTagsHis-TEV-SUMO-ExpressionBacterialMutationPromoterT5Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only