Addgene

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pU6 SgRNA GAPDH

Plasmid

#162736

Purpose

Mammalian expression

Depositor

Amit Choudhary

Article

Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

Insert

Spacer sequence for GAPDH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (GAPDH)

Plasmid

#170120

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (GAPDH Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-CC

Plasmid

#225476

Purpose

Express mEGFP-GAPDH-CC fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with CC2 domain of ROCK2 (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-EGFP

Plasmid

#115918

Purpose

donor to insert IRES-EGFP at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-EGFP

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(-)-GAPDH-IRES-dTomato

Plasmid

#115919

Purpose

donor to insert IRES-dTomato at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-dTomato

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-R rich

Plasmid

#225480

Purpose

Express mEGFP-GAPDH-Rrich fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with R-rich region of TDRD3 (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor

Plasmid

#225479

Purpose

Express mEGFP-GAPDH-Tudor fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-RGG

Plasmid

#225477

Purpose

Express mEGFP-GAPDH-RGG fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with RGG domain of TOP3B (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-GAPDH-IRES-dTomato

Plasmid

#115912

Purpose

donor to insert IRES-dTomato at the GAPDH locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

IRES-dTomato

Use

Donor template

Tags

dTomato

Expression

Mutation

Promoter

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with interspersed loops (GAPDH)

Plasmid

#180183

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with interspersed loops (GAPDH Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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KI(GAPDH)-P2A-EGFP-CMV-NeoR

Plasmid

#114009

Purpose

HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection

Depositor

Pavel Volchkov

Article

Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126

Inserts

GAPDH left arm (GAPDH Chicken)
eGFP
NeoR
GAPDH right arm

Use

Tags

Expression

Mammalian

Mutation

Promoter

-, CMV, and endogenous promoter (chicken GAPDH in…

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor-R rich

Plasmid

#225478

Purpose

Express mEGFP-GAPDH-Tudor-R fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 with 8 bp bulge loops (GAPDH)

Plasmid

#170121

Purpose

AAV vector carrying a guide RNA targeting the human GAPDH mRNA

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA with 8bp bulge loops (GAPDH Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191156

Purpose

Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP-3xMS2

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

Plasmid

#191177

Purpose

Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (frameshifted)

Use

Tags

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#191178

Purpose

Mammalian expression of EK0493 with second stop codon.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (double-stop)

Use

Tags

Expression

Mammalian

Mutation

additional stop codon

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19_GZG

Plasmid

#117226

Purpose

shble flanked by an endogenous GAPDH promoter and terminator system

Depositor

Jackie Collier

Insert

shble

Use

Thraustochytrid expression

Tags

Expression

Mutation

Promoter

GAPDH

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Tags

Expression

Mutation

Promoter

GAPDH

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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Talin-1 WT

Plasmid

#201798

Purpose

Expresses Talin-1 protein in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Talin-1 (Tln1 Mouse)

Use

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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SZ_EC_02

Plasmid

#155341

Purpose

Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR

Depositor

Jens Nielsen

Article

Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:

Inserts

tef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator

Use

Easycloneyali insert vector

Tags

Expression

Yeast

Mutation

Promoter

gapdh, pyk1, and tef1

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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