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Cdc2-DN-HA
Plasmid#1889DepositorInsertCdc2 (CDK1 Human)
UseTagsHAExpressionMammalianMutationD146N, dominant negativePromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pLIB-GST-3C-CDK1
Plasmid#177011PurposeGenerate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)DepositorInsertCDK1 (CDK1 Human)
UseTagsGST-3CExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1-P2A-Hygro_Barcode
Plasmid#170221PurposeBarcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode
Plasmid#170220PurposeBarcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMutationT14A, Y15FPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF1145
Plasmid#141799PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CDK1 gRNA (BRDN0001147731)
Plasmid#77388Purpose3rd generation lentiviral gRNA plasmid targeting human CDK1DepositorInsertCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK1 gRNA (BRDN0001149185)
Plasmid#77387Purpose3rd generation lentiviral gRNA plasmid targeting human CDK1DepositorInsertCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK1 gRNA (BRDN0001149272)
Plasmid#77390Purpose3rd generation lentiviral gRNA plasmid targeting human CDK1DepositorInsertCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3050
Plasmid#144526PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3051
Plasmid#144527PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3049
Plasmid#144525PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCDK1 (CDK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CDK1 gRNA (BRDN0001146007)
Plasmid#77389Purpose3rd generation lentiviral gRNA plasmid targeting human CDK1DepositorInsertCDK1 (CDK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1
Plasmid#177330PurposeCo-expression of the human active Cdk1/cyclin B1 kinase complexDepositorUseTags2 x Strep-tag and 8 x HisExpressionInsectMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CDC2
Plasmid#23776DepositorInsertCDC2 (CDK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag CDC2
Plasmid#20446DepositorInsertCDC2 (CDK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1/Cks1
Plasmid#177329PurposeCo-expression of the human active Cdk1/cyclin B1/Cks1 kinase complexDepositorInsertsUseTags2 x Strep-tag and 8 x HisExpressionInsectMutationPromoterp10 and polhAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only
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