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Plasmid#175571PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)
Plasmid#175572PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR
Plasmid#170808PurposePiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistanceDepositorInsertsUseTagsExpressionMammalianMutationSynonymous mutation in PYL1 to remove XhoI site (…PromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE
Plasmid#192161PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGW05 AAV-CRISPRa base vector pAAV-OE_U6-Lib(MS2)-EF1a-MPH-sPA
Plasmid#192159PurposeAAV-CRISPRa base vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCK389.AAV
Plasmid#177327PurposeaTc inducible MCP-SoxS double mutant expression for CRISPRa in E.coli. Contains constitutive dCas9 and scRNA expressionDepositorInsertsSoxS
dCas9
UseCRISPRTagsMCP (MS2 Coat Protein)ExpressionMutationR93A,S101APromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLY153
Plasmid#130951Purposereporter circuit (PpspA-2G6 with sfgfp::ASV)DepositorInsertsfgfp
UseSynthetic BiologyTagsASV tagExpressionBacterialMutationPromoterPpspA-2G6Available sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
S5_EcoRV_Ef1a_AscI
Plasmid#128469PurposeEf1a promoterDepositorInsertEf1a promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEP179_pX330K
Plasmid#137882PurposeEntry vector for multi-gRNA cloningDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA303.DA9.1B.mRFP
Plasmid#206802PurposemRFP with synthetic promoter HP3 (from Figure S21)DepositorInsertmRFP
UseTagsExpressionMutationPromoterDA9.1B (HP3)Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDA010.EL222
Plasmid#206801PurposeJ23106.EL222DepositorInsertEL222
UseTagsExpressionMutationPromoterJ23106Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJF143.J3
Plasmid#153028PurposeJ3-mRFP1DepositorInsertJ3-mRFP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA040.BLD7-mRFP
Plasmid#206800PurposeEL222_D7_mRFPDepositorInsertBLD7.mRFP
UseTagsExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only