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Plasmid#17518DepositorInsertB. subtilis DEAD-box helicase YxiN
UseTagsintein-chitin binding domainExpressionBacterialMutationE152Q mutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS1C
Plasmid#55168PurposeEmpty vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PxylA
Plasmid#55178PurposeXylose-inducible promoterDepositorInsertxylA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterxylAAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PliaI
Plasmid#55177PurposeBacitracin-inducible promoterDepositorInsertliaI promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterliaIAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PliaG
Plasmid#55174PurposeConstitutive promoterDepositorInsertLiaG promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
J23101
Plasmid#55176PurposeVery weak constitutive promoterDepositorInsertJ23101 promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterJ23101Available sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL003
Plasmid#215335PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterhyper-pSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterPvegAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG-A-sacB(SP)-B
Plasmid#87370PurposeAn ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assemblyDepositorInsertSacB signal peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG-A-celA(SP)-B
Plasmid#87371PurposeAn ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assemblyDepositorInsertCelA signal peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG-A-xynA(SP)-B
Plasmid#87372PurposeAn ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assemblyDepositorInsertXynA signal peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)-RNase HIII
Plasmid#12683DepositorInsertRNase HIII
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOSV00157
Plasmid#222937PurposeKill switch plasmid for DNA sensor construction using Bacillus subtilisDepositorInsertTxpA-RatA toxin-antitoxin
UseTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-479.wt
Plasmid#17516DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206PromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.E152Q
Plasmid#17517DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479; E152Q mutat…PromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS3Clux
Plasmid#55172Purposelux-reporter vector, integration at sacA, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS2E
Plasmid#55169PurposeEmpty vector, integration at lacA, ampr, mlsrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoternoneAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only