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Addgene
Showing: 51 - 100 of 222 results
  1. pHflu2

    Plasmid
    #203880
    Purpose
    pHI1 with insertion of ampicillin-resistance gene
    Depositor
    Insert
    broad-spectrum class A beta-lactamase TEM-116
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pHflu3

    Plasmid
    #203881
    Purpose
    pHI1 with insertion of kanamycin-resistance gene
    Depositor
    Insert
    aminoglycoside O-phosphotransferase APH(3')-IIa
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pJL1-PD-DQNAT

    Plasmid
    #198216
    Purpose
    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Insert
    PD-DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pAIP4_5EF9

    Plasmid
    #234154
    Purpose
    Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli
    Depositor
    Insert
    5EF9 (PB2 Influenza B virus (B/Lee/1940))
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

    Plasmid
    #175020
    Purpose
    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP
    Depositor
    Insert
    D/bovine/Nebraska/9-5/2012
    Use
    Tags
    GCN4-TEV-sfGFP-TwinStrep
    Expression
    Mammalian
    Mutation
    s57a esterase knock out mutant
    Promoter
    CMV
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pCSThaP6ERT(#184)

    Plasmid
    #184103
    Purpose
    cyclofen-inducible mouse Pax6 activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Insert
    HA-MmPax6-ERT2
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6+T7
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pCSZPKIamutHAERT(#119)

    Plasmid
    #184076
    Purpose
    cyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Insert
    ZfmutPKAinhiba-HA-ERT2
    Use
    Synthetic Biology
    Tags
    internal tag: HA
    Expression
    Mammalian
    Mutation
    ZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…
    Promoter
    sCMV+SP6
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pCSZPKIaHAERT(#118)

    Plasmid
    #184075
    Purpose
    cyclofen-inducible PK inhibitor alpha (wt) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Insert
    ZfPKAinhiba-HA-ERT2
    Use
    Synthetic Biology
    Tags
    internal tag: HA
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. HA Display H3_A/Hong Kong/1/1968

    Plasmid
    #206506
    Purpose
    Expresses H3_Hong Kong/1/1968 in the surface of mammalian cells
    Depositor
    Insert
    H3_A/Hong Kong/1/1968
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    deleted amino acids 1651 to 1735
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pT22i5uasH3En2SE4P3Che(#1212)

    Plasmid
    #184098
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic
    Depositor
    Insert
    3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    3xHA
    Expression
    Mutation
    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…
    Promoter
    5xUAS
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 51 - 100 of 222 results