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Plasmid#213002Purposeexpresses human P53DepositorInsertP53 (TP53 Human)
UseTagsExpressionMammalianMutationS46DPromotercmvAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBOBI TP53 S46A
Plasmid#213001Purposeexpresses human P53DepositorInsertP53 (TP53 Human)
UseTagsExpressionMammalianMutationS46APromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgTp53#3/Cre
Plasmid#173622PurposeExpresses a Tp53-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Tp53 (Trp53 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TK-Firefly-cjTP53RK
Plasmid#122283PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjTP53RK 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-deltaNp53:WTp53
Plasmid#49244Purposeexpresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cellsDepositorInsertp53 deltaN and p53 WT (TP53 Human)
UseTagsHis and IRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-TP53RK_IDG-K
Plasmid#170589PurposeGateway destination clone of TP53RK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-TP53RK (TP53RK Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E352 Hs.TP53-nostop
Plasmid#70636PurposeGateway ORF clone of human TP53 [NM_000546.5] without stop codon (for C-terminal fusions)DepositorInsertTP53 (TP53 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-pro-siRNA-V2-TP53
Plasmid#111089PurposepET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.DepositorInsertTP53 (TP53 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAav-TP53-T2A-BirA*
Plasmid#115652PurposerAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassetteDepositorUseAAVTagsBirA* and T2AExpressionMutationHomology region 1 and Homology region 2PromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Control-TP53-LRG-GFP
Plasmid#225874PurposeGuides targeting P53 and a control sequenceDepositorInsertControl and TP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
RB1-TP53-LRG-GFP
Plasmid#225876PurposeGuides targeting TP53 and RB1DepositorUseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-T7-hTP53-P0~670-FLuc
Plasmid#208640Purposefor in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.DepositorInsertTP53, UTR and partial CDS (TP53 Human)
UseLuciferase; In vitor transcription templateTagsFirefly Luciferase (F-Luc) and T7 promoterExpressionMutationUTR and partial CDS in-frame with F-LucPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAav-TP53-PQS1-3xFLAG
Plasmid#84884PurposerAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertsUseAAVTagsPQS1 3xFLAGExpressionMutationPromoterno and noAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
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MiniCoopR U6:gRNA p53, mitfa:Cas9
Plasmid#118841PurposeTargets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytesDepositorInserttp53 gRNA (tp53 Zebrafish)
UseCRISPR; Tol2TagsExpressionMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD (TET)
Plasmid#214083PurposeMammalian expression tetramerization domain (TET) of human TP53DepositorInserthP53DD (TP53 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD (TET+CTD)
Plasmid#214082PurposeMammalian expression tetramerization domain (TET) and C-terminal negative regulatory domain (CTD) of human TP53DepositorInserthP53DD (TP53 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53
Plasmid#11653DepositorInsertGFP, shRNA against TP53 (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p53 R175H
Plasmid#39480DepositorInsertTP53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationP72R and R175HPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pET23 P53DBD
Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
UseTagsnoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVUHshp53-tTR-KRAB
Plasmid#11654DepositorInserthUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCSP1-mp53AS
Plasmid#20896DepositorInsertp53AS (Trp53 Mouse)
UseTagsExpressionMammalianMutation5' natural EcoRV and SacI restriction sites …PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCI-Neo-p53mut
Plasmid#99239PurposeMutant p53 encoded by AS-30D rat hepatoma cell lineDepositorInsertp53 (Tp53 Rat)
UseTagsExpressionMammalianMutationSilent mutations in translated protein at Gly (10…PromoterCMV immediate/early enhancer/promoterAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
145-pGL3ctrl-3' UTR
Plasmid#28175DepositorInsertLuciferase flanked by tp53 UTRs (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationNote that insert length is based on sum of the 2 …PromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R175H
Plasmid#22936DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR175H MutantPromoterAvailable sinceMarch 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R273H
Plasmid#22934DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR273H MutantPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R249S
Plasmid#22935DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationR249S MutantPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD
Plasmid#214080PurposeMammalian expression of human P53DDDepositorInserthP53DD (TP53 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_R280K
Plasmid#22933DepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsv5ExpressionMammalianMutationR280K mutantPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 tethered
Plasmid#49245Purposeexpresses two human WT p53s tethered together and also expresses EGFP in mammalian cellsDepositorInsertp53-p53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCE-mp53DD
Plasmid#41856PurposeNon-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragmentDepositorInsertTrp53 (Trp53 Mouse)
UseTagsExpressionMammalianMutationΔ40-903PromoterCAGAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti6/V5-p53_wt p53
Plasmid#22945Purpose3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tagDepositorInserttumor protein 53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
T7-p53DD-pcDNA3
Plasmid#25989DepositorInserttumor protein p53 (TP53 Human)
UseTagsT7ExpressionMammalianMutationtruncated cDNA encodes truncated protein (aa 300-…PromoterAvailable sinceOct. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mp53DD
Plasmid#41859PurposeNon-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragmentDepositorInsertTrp 53 (Trp53 Mouse)
UseTagsExpressionMammalianMutationΔ40-903PromoterCAGAvailable sinceJuly 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDF RBP-FL P53
Plasmid#174042PurposeExpress Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)DepositorInsertP53 gene (TP53 Human)
UseTagsThermoanaerobacter Tencongenesis ribose binding p…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSP1-mp53AS-6xMUT
Plasmid#20904DepositorInsertp53AS (Trp53 Mouse)
UseTagsExpressionMammalianMutationpoint mutation (Ser/Thr-Ala) of the 6 N-terminal …PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-hP53DD
Plasmid#214084PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-hP53DDDepositorInsertPEmax-P2A-hP53DD (TP53 Synthetic, Human, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKan cpRBP-FL P53
Plasmid#174504PurposeExpress Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)DepositorInsertP53 gene (TP53 Human)
UseTags20 amino acid linker containing HRV3C protease si…ExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pLX313-Firefly luciferase
Plasmid#118017PurposeConstitutive expression of Firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Renilla luciferase
Plasmid#118016PurposeConstitutive expression of Renilla luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF1252
Plasmid#143778PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1253
Plasmid#142764PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1261
Plasmid#143779PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1262
Plasmid#142772PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1260
Plasmid#142771PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1259
Plasmid#142770PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only