-
Plasmid#81207Purposefor targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 5' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch12_62418577-gRNA-for
Plasmid#81216PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-for1
Plasmid#81210Purposefor targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 3' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-rev1
Plasmid#81208Purposefor targeting Ch10 psuedo gix site in PCDH15 gene(six base pairs from the cas9 site and 5' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-BACH1
Plasmid#232269PurposeExpression of N-terminal tagged HA-BACH1 (H. sapiens) in human cell lines.DepositorInsertBACH1 (BACH1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL1-V3-FETCH1
Plasmid#210650PurposeIn vitro expression of V3-FETCH reporter from the T7 promoterDepositorInsertV3-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1-L24_sfGFP
Plasmid#207865PurposeYeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZ3CH10-donor
Plasmid#176368PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZ3CH10
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCh1809_pBS_cdc42E6-boxB
Plasmid#118609Purposecdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldownDepositorInsertcdc42E6 3'UTR (Cdc42 Mouse)
UseIn vitro transcriptionTagsboxBExpressionMutationPromoterT3Available sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh1807_pBS_cdc42E7-boxB
Plasmid#118612Purposecdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldownDepositorInsertcdc42E7 3'UTR (Cdc42 Mouse)
UseIn vitro transcriptionTagsboxBExpressionMutationPromoterT3Available sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CH110: Plas_lac_bD
Plasmid#104867PurposeCH110: Plas_lac_bDDepositorInsertHybrid Promoter : Plas_lac_bD
UseTagsExpressionBacterialMutationPromoterHybrid Promoter : Plas_lac_bDAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHU6_gRNA_Ch10-for2
Plasmid#81209Purposefor targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)DepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1
Plasmid#159130Purposeexpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 Bach1 S990A
Plasmid#73643PurposeMammalian expression of Bach1 S990A mutant (BRCA binding mutant).DepositorInsertBach1 (BACH1 Human)
UseTagsHis and MycExpressionMammalianMutationS990A (BRCA binding mutant)PromoterCMVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCH1 - pBait-empty
Plasmid#174663Purposearabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
DRH335: FCK-Optopatch1
Plasmid#51695PurposeCoexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiologyDepositorInsertOptopatch1
UseLentiviralTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS mNotch1-IC (DM#270)
Plasmid#15079DepositorInsertNotch1-IC (Notch1 Mouse)
UseTagsExpressionBacterialMutationK1750APromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-Notch1 3'UTR
Plasmid#84595PurposeRenilla luciferase reporter containing NOTCH1 3' UTRDepositorInsertNOTCH1 3'UTR (NOTCH1 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMutationPromoterCMVAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
anti-Notch1_E6-pBIOCAM5
Plasmid#39344DepositorUseTags3xFLAG, 6xHis, and human FcExpressionMammalianMutationPromoterCMVAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJL1-FETCH1-StrepII
Plasmid#210648PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH1
UseSynthetic BiologyTagsStrep-TagIIExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-FL-TagBFP
Plasmid#120921PurposeExpresses BFP tagged Ptch1DepositorInsertptch1 (Ptch1 Mouse)
UseTagsTagBFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F
Plasmid#159132PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11FPromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Notch1 del-NRR
Plasmid#188353PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-del-NRR-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_Notch1-NRRdeletion-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch1#1/Cre
Plasmid#193223PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 geneDepositorInsertsgNotch1#1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-GFP
Plasmid#120903PurposeExpresses Ptch1-B for function assaysDepositorInsertptch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH1h-1
Plasmid#105856PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG1-derived CH1 and hinge.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG3 with CH1 and hin…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_CH1h-3
Plasmid#105850PurposeExpression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH1 and hinge.DepositorUseTagsExpressionMammalianMutationhybrid heavy chain of mouse IgG1 with CH1 and hin…PromoterhEF1-HTLV promAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVLAD6-mmPtch1-B-SBP
Plasmid#120886PurposeBacMam vector for Ptch1-B expressionDepositorInsertptch1 (Ptch1 Mouse)
UseTagsSBPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch13_102010574-gRNA-rev
Plasmid#81219PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch13 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-L235C-HA
Plasmid#120906PurposeExpresses mmPtch1-B (L235C)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, L…PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-T1404A
Plasmid#116615PurposeLentiviral expression of PTCH1 T1404ADepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationT1404APromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-TagBFP
Plasmid#120910PurposeExpresses BFP tagged Ptch1-BDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch12_62418577-gRNA-rev
Plasmid#81217PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS cDach1 (CT#319)
Plasmid#13959DepositorInsertDachshund1 (DACH1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FFSS-BACH1
Plasmid#232273PurposeExpression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell linesDepositorInsertBACH1 (BACH1 Human)
UseTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-FETCH1
Plasmid#210646PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH1
UseSynthetic BiologyTagssuperFLAG (DYKDEDLL)ExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-mNF-BACH1
Plasmid#199219PurposeLPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection.DepositorInserthTetR::P2A::EGFP::BACH1 (BACH1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV D2ir promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-shBACH1_BFP
Plasmid#199217PurposeExpression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.DepositorInsertshBACH1 (BACH1 Synthetic)
UseRNAiTagsExpressionMutationPromoterhuman U6Available sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch1#2/Cre
Plasmid#193224PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 geneDepositorInsertsgNotch1#2 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A
Plasmid#159131PurposeExpresses BACH1 in mammalian cellsDepositorInsertBACH1 (BACH1 Human)
UseTags2X-FLAG _ 2X-STREPExpressionMammalianMutationY11APromoterCMVAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOZ-FH-C Bach1 S990A
Plasmid#73647PurposeRetroviral expression of Bach1 S990A mutant (BRCA binding mutant).DepositorInsertBach1 (BACH1 Human)
UseRetroviralTagsFLAG and HisExpressionMammalianMutationS990A (BRCA binding mutant)PromoterunknownAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH115gp160-754*
Plasmid#123281PurposeMammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (CH115) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH111gp160-754*
Plasmid#123263PurposeMammalian expression plasmid for Env from the CH111 HIV-1 isolate; C-terminal truncationDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH115gp160-QES.c14
Plasmid#123279PurposeMammalian expression plasmid for Env from the CH115 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertHIV-1 (CH115) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations V208M, …PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-D894N
Plasmid#116611PurposeLentiviral expression of PTCH1 D894NDepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationD894NPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-G1212D
Plasmid#116612PurposeLentiviral expression of PTCH1 G1212DDepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationG1212DPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-R1308Kfs*64
Plasmid#116613PurposeLentiviral expression of PTCH1 R1308Kfs*64DepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationR1308Kfs*64PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-S1203Rfs*52
Plasmid#116614PurposeLentiviral expression of PTCH1 S1203Rfs*52DepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationS1203Rfs*52PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PTCH1-T768I
Plasmid#116616PurposeLentiviral expression of PTCH1 T768IDepositorInsertPTCH1 (PTCH1 Human)
UseLentiviralTagsExpressionMutationT768IPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-Y233C-HA
Plasmid#120904PurposeExpresses Ptch1-B (Y233C) for function assaysDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, Y…PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only