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VAMP8-SuperNova

Plasmid

#131855

Purpose

Expresses SuperNova fused to endosomal SNARE VAMP8

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Use

Tags

Myc-tag and VAMP8 (mouse sequence)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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NOVA1_Full-length_pGEX

Plasmid

#135147

Purpose

Recombinant expression of NOVA1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NOVA1 (NOVA1 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CNC3 (CASANOVA-C3)

Plasmid

#137191

Purpose

Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

Insert

CNC3 (CASANOVA-C3)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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2MLS-HyperNova/pcDNA3

Plasmid

#221457

Purpose

Expressing HyperNova in mitochondria

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Calreticulin-SuperNova-KDEL

Plasmid

#131857

Purpose

Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Use

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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SuperNova Green/ pRSETB

Plasmid

#124839

Purpose

bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

Depositor

Takeharu Nagai

Article

Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

Insert

SuperNova Green

Use

Tags

No tag

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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MPC020: CamKII CMV_NovArch_citrine

Plasmid

#153193

Purpose

Encodes Novarch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

Tags

citrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CASANOVA

Plasmid

#113036

Purpose

AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-Nova1-GFP

Plasmid

#217033

Purpose

Overexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neurons

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Nova1 (Nova1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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