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Plasmid#62209PurposeExpresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.DepositorInsertcas9
UseTagsExpressionInsectMutationPromoteract5CAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
PRExpress-Bxb1-hsp70pA
Plasmid#158391Purposeheat shock inducible Bxb1 integrase for drosophila melanogasterDepositorInsertBxb1
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS-Ceru-10unit
Plasmid#158389Purpose10-unit intMEMOIR recording array for Drosophila melanogasterDepositorInsert10unit recording array
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo
Plasmid#140166PurposeExpresses a regulatable secretory cargo for Drosophila melanogasterDepositorInsertssBip-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT17
Plasmid#204298PurposeFor introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.DepositorInsert3XP3-EYFP
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6
Plasmid#176652PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4B
Plasmid#176665PurposeExpression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLib6.6B
Plasmid#176666PurposeExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprTagsExpressionInsectMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-WingGFP-tan
Plasmid#83473PurposeEGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsertWingGFP-tan
UseCRISPRTagsExpressionBacterial and InsectMutationPromoterHsp70 and enhancer from D. melanogaster yellow ge…Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASt-ssBiP-ESCargo*
Plasmid#140165PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogasterDepositorInsertssBip-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionInsectMutationPromoterhs70Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP78 pUAST Orco mRNA
Plasmid#53431PurposeExpresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS controlDepositorInsertOrco (Orco )
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster nanosAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster vasaAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM60
Plasmid#154159PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel1A-specifi…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#154163PurposeFirefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation20 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWalium Htt19Q-mCherry
Plasmid#201248Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertHuntingtin (HTT Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST ɑSynWT-EGFP
Plasmid#201249Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertɑ-Synuclein (SNCA Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAA2
Plasmid#154162PurposeFirefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation16 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAA1
Plasmid#154161PurposeFirefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation12 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only