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Showing: 3961 - 3980 of 8424 results

  4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  8. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110411-plasmid-map-sequence-id-213311

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  10. M-tdTom-SP

    Plasmid
    #48677
    Purpose
    Mammalian tdTomato activation reporter for SP with GTCCCCTCCACCCCACAGTG protospacer
    Depositor
    George Church
    Article
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Insert
    TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Sp6
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pMZ288

    Plasmid
    #59897
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59897-plasmid-map-sequence-id-94870

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  17. pMZ289

    Plasmid
    #59898
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210
    Depositor
    Mikel Zaratiegui
    Article
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59898-plasmid-map-sequence-id-94871

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Showing: 3961 - 3980 of 8424 results