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Plasmid#72064PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.30
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.22 EC10-AP-His
Plasmid#72060PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.22
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-AP-His
Plasmid#72061PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.23
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(S)-AP-His
Plasmid#72001PurposeExpresses the N-terminal extracellular region of the PlexinB1 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.22 EC10-Fc-His
Plasmid#72186PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.22
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-INSR-V5/HIS
Plasmid#201980Purposeexpression of human INSR receptor tyrosine kinase in mammalian cellsDepositorInsertINSR receptor tyrosine kinase (INSR Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
Plasmid#108833PurposeExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDsDepositorInsertFlp recombinase fragment aa28 to aa396
UseCre/Lox and Synthetic BiologyTagsABI,NLS and GAIExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLINK cyclin T1 (1-280) myc (K93D) (P#580)
Plasmid#14611DepositorInsertcyclin T1 (1-280) K93D (CCNT1 Human)
UseTagsmycExpressionMammalianMutationK93D. Deficient in binding to CDK9. Inactive in 3…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJT305 Pa01 Lenti pEF-attB LSR GFP
Plasmid#184948PurposeLentiviral delivery of recombinase landing padDepositorInsertPa01 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2Cre
Plasmid#82415PurposeLentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning siteDepositorInsertCre Recombinase
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationMutated BsmBI cut sitePromoterEFS (P2A)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2281
Plasmid#111902PurposessAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle346 (NEFM Human)
UseAAVTagsExpressionMutationPromoterPle346 (NEFM MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST53-LRRK2-WT
Plasmid#25044DepositorInsertLRRK2 (LRRK2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pBabe-puro
Plasmid#19005DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF1alpha P402A/P564A-pcDNA3
Plasmid#18955DepositorInsertHypoxia inducible factor 1 alpha (HIF1A Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 402 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T286D/T305A/T306A)
Plasmid#127392PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D/T305A/T306A)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 286 to Aspartic acid and Threon…PromoterpCAGAvailable sinceJuly 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-importinα
Plasmid#119679PurposeExpresses DsRed-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsDsRedExpressionMammalianMutationContains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MEF2A-Sensor
Plasmid#208928Purposein vitro MAPK-Sensor for luminescent detection of ERK and p38-activityDepositorInsertMef2a docking sequence+Phospho site (MEF2A Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-P405A/P531A-pcDNA3
Plasmid#18956DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…PromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ALK-V5/HIS
Plasmid#200115PurposeExpression of human ALK receptor tyrosine kinase in mammalian cellsDepositorInserthuman ALK receptor tyrosine kinase (ALK Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikkb-FLAG WT
Plasmid#23298DepositorInsertIkkbeta (IKBKB Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only