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Plasmid#85450PurposeExpress SpCas9 in mammalian cellsDepositorInsertpRSV
UseAAVTagsExpressionMutationPromoterpRSVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAsp-AMA-gpdA-ptr
Plasmid#191016PurposeCRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)DepositorTypeEmpty backboneUseUnspecified; AspergillusTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGRB
Plasmid#71539Purposefor expression of gRNA in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ231196Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-ultraID-Blast
Plasmid#207715PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-mScarlet-Blast
Plasmid#207711PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLH-sgRNA1-2XPP7
Plasmid#75390PurposesgRNA1-2XPP7DepositorInsertsgRNA1-2XPP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mScarlet
Plasmid#207678PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti
Plasmid#138315PurposeLenti expression of mTgBFP2DepositorInsertmTAGBFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459_gRNA-AAVS1_hspCas9
Plasmid#193309PurposeCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)DepositorInsertCas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
UseCRISPRTagsExpressionMammalianMutationnot applicablePromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA2 TKIT donor
Plasmid#169421PurposeDonor DNA for TKIT knockin of SEP to GluA2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYZ463
Plasmid#187971PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-ultraID
Plasmid#207693PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJAT62
Plasmid#204301PurposeExpress phiC31 integrase under heat shock control. Insert marked with ie1-EGFP, which expresses in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330A-1x3
Plasmid#58767PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-miniTurbo-Puro
Plasmid#207725PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-SEP-GluA1 TKIT
Plasmid#169441PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-V5-Puro
Plasmid#207730PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-mNeon
Plasmid#207676PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only