A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger.
Chang PK
Microbiol Spectr. 2023 Feb 14;11(1):e0464822. doi: 10.1128/spectrum.04648-22. Epub 2023 Jan 18.
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PubMed
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
191015 | pAf-CRISPR-yA | CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker. |
191016 | pAsp-AMA-gpdA-ptr | CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi) |