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pBABEpuro GFP-LC3

Plasmid

#22405

Purpose

retroviral expression of GFP-tagged LC3

Depositor

Jayanta Debnath

Article

Fung et al Mol Biol Cell. 2008 Mar . 19(3):797-806.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-Paxillin EGFP

Plasmid

#187688

Purpose

In vivo visualization of Paxillin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Paxillin (PXN Chicken)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-BclXL-pEGFP-C1

Plasmid

#177409

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bcl-XL.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

Bcl-XL

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pC035 - dLwCas13a-msfGFP

Plasmid

#91925

Purpose

Expresses catalytically inactive LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS-3xHA and NLS-msfGFP

Expression

Mammalian

Mutation

R488A and R1060A

Promoter

EF-1a

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-GFP

Plasmid

#190231

Purpose

AAV vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-YKT6

Plasmid

#116946

Purpose

Expressing GFP-YKT6 for mammalian cells

Depositor

Noboru Mizushima

Article

Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

Insert

Synaptobrevin homolog YKT6 (YKT6 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RUNX1-WT

Plasmid

#194574

Purpose

Mammalian Expression of mEGFP-RUNX1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR GFP-CaaX

Plasmid

#113020

Purpose

for lentivirus production of GFP-CaaX

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

GFP-CaaX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-GFP-PKHD1

Plasmid

#108408

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

Depositor

Gregory Germino

Article

Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

Insert

PKHD1 (PKHD1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(deltaNC)

Plasmid

#110190

Purpose

Expression of mouse MeCP2 (deltaNC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSP4-E228Q

Plasmid

#80351

Purpose

Expression of GFP-tagged, dominant negative VPS4 mutant

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

VPS4-E228Q (VPS4A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-C1-PKCgamma-C1A

Plasmid

#21205

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKCgamma-C1A (Prkcg Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

PKCgamma-C1A domain. (Lab plasmid WC 0018)

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pdCas9-DNMT3A-EGFP

Plasmid

#71666

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldoš

Article

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, Human, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP Mer

Plasmid

#14998

Depositor

Raymond Birge

Article

Wu et al J Cell Sci. 2005 Feb 1. 118(Pt 3):539-53

Insert

Mer (Mertk Mouse)

Use

Tags

IRES EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-mRFP

Plasmid

#128126

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen (benzoate responsive promoter)

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact EGFP

Plasmid

#187686

Purpose

In vivo visualization of actin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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