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Plasmid#85823PurposeExpressed C-term tagged APEX2 on HMOX1 in mammalian cellDepositorInsertHMOX1-V5-APEX2 (HMOX1 Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_V5-APEX2-Sec61B
Plasmid#83412PurposeExpressed N-term tagged APEX2 on Sec61B in mammalian cellDepositorInsertV5-APEX2-Sec61B (SEC61B Synthetic, Human)
UseTagsAPEX2 and V5ExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e(TadA V106W)
Plasmid#138509PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSaCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCVhygro-F-Ezh2-F667I
Plasmid#24927DepositorInsertEzh2 (EZH2 Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationPhenylanaline(F)667 mutated to Isoleucine(I)PromoterAvailable sinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTS99 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-3C-MCS
Plasmid#199352PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP
Plasmid#199364PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
∆C' terminal-DPYSL2-pLJC2
Plasmid#189867PurposeExpresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cellsDepositorInsertΔC-DPYSL2
UseLentiviralTags3X FLAGExpressionMammalianMutationdeleted the C'terminus of DPYSL2 (491-572 aa)PromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
psicheck-2-Casp8
Plasmid#48172Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame elementDepositorInsert5" UTR of CASP8 (CASP8 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_mKate2
Plasmid#96942PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertfar-red fluorescent protein
UseLentiviralTagsExpressionMutationE87D (please see depositor comments below)PromoterCaMKIIaAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-PKCg-mEGFP-N1
Plasmid#112270PurposeIDOCKSgamma Donor ConstructDepositorInsertprotein kinase c gamma (Prkcg Mouse)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGoldyTALEN-VIM-TAL-2
Plasmid#62852PurposeTALEN mediated gene editing at human VIM locusDepositorInsertTALEN array
UseTALENTagsExpressionMammalianMutationPromoterminiCAGGAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGoldyTALEN-VIM-TAL-1
Plasmid#62855PurposeTALEN mediated gene editing at human VIM locusDepositorInsertTALEN array
UseTALENTagsExpressionMammalianMutationPromoterminiCAGGAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP
Plasmid#199368PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-ALFA-P2A-TagBFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTracer_Cr-TRP1
Plasmid#64881PurposeExpresses CrTRP1 in mammalian cellsDepositorInsertTransient receptor potential 1
UseTagseGFPExpressionMutationPromoterCMVAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTW102
Plasmid#115945PurposeORF insert for replicon MoCloDepositorInsertLvl0 DDd-L7Ae
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 KD
Plasmid#13322DepositorInsertPINK1 kinase domain (PINK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 112-496; Kinase dead: K219D/D362A/D3…PromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
LbABE8e(TadA-8e V106W)
Plasmid#138508PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-dLbCas12a
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Promoter-4xBS_BHLHE40
Plasmid#153064PurposeFour BHLHE40 consensus binding sites cloned upstream of luciferase reporter geneDepositorInsert4X consensus BHLHE40 binding sites
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABE8e-dimer
Plasmid#138498PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Beta1b mCherry
Plasmid#89892PurposeMammalian expression of Beta1b mCherryDepositorInsertCacnb1 (Cacnb1 Rat)
UseTagsmCherryExpressionMammalianMutationmCherry sequence inserted just before the Stop co…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only