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Plasmid#92225PurposeExpression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue Y35 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP Double
Plasmid#100729PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motif and …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta2(616-951)-GFP ∆CBM
Plasmid#100728PurposeTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoform, deletion of clathrin box motifPromoterCMVAvailable sinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
JAM3-bio-His
Plasmid#51807PurposeExpresses full-length Junctional adhesion molecule C precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertJAM3 (JAM3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rabbit Cav 2.2 W391A pMT2
Plasmid#58734Purposeexpression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2DepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP∆678-681-shTACC3
Plasmid#69115PurposeDual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationDeletion of 678-681aa region. Silent mutations i…PromoterCMVAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
STIM1-bio-His
Plasmid#51830PurposeExpresses full-length Stromal interaction molecule 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSTIM1 (STIM1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ENTP-bio-His
Plasmid#53358PurposeExpresses full-length Ectonucleoside triphosphate diphosphohydrolase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertENTP (ENTPD1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
C20-His-bio
Plasmid#52327PurposeExpresses full-length Adipocyte plasma membrane-associated protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertC20 (APMAP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1mLCAD_8KR_FLAG
Plasmid#24485DepositorInsertLong chain acyl-CoA dehydrogenase (Acadl Mouse)
UseTagsFLAGExpressionMammalianMutationLys42, 156, 189, 240, 254, 318, 322, 358Arg (8KR)PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-CERT(PHD)(W33A)
Plasmid#221761PurposeStable expression of GFP-CERT(PHD)(W33A) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–116, mutated 33W to 33APromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TRPML1(PHD)
Plasmid#221752PurposeStable expression of GFP-TRPML1(PHD) in mammalian cellsDepositorInsertTRPML1 (MCOLN1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TRPML1(PHD) (amino acids 1–69)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0043-PspCas13b-gRNA LINE1
Plasmid#223699PurposegRNA of dPspCas13b-FTO targeting LINE1DepositorInsertgRNA target sequence for LINE1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGhMeCP2 (pc3972)
Plasmid#212033PurposeExpresses human MeCP2 tagged N-terminal to Halo-tagDepositorInsertMeCP2 (MECP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-STAT4-P2A-mCherry
Plasmid#203853PurposeTet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertSTAT4 (STAT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 W391A pcDNA3
Plasmid#206085Purposeexpression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-UFBP1 M5
Plasmid#204563PurposeExpresses GST-tagged UFBP1 M5 in E.coliDepositorInsertUFBP1 (DDRGK1 Human)
UseTagsExpressionBacterialMutation215-314aaPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-N3-CYB5R3 deltaN26
Plasmid#204568PurposeExpresses EGFP-tagged CYB5R3 deltaN26 in mammalian cellsDepositorInsertCYB5R3 (CYB5R3 Human)
UseTagsExpressionMammalianMutationdelta2-26aaPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only