Addgene

We narrowed to 11,759 results for: CAR

Showing: 3921 - 3940 of 11759 results

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pCD294.9

Plasmid

#113319

Purpose

dCas9, PCP-SoxS_R93A, J109 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a1-479.wt

Plasmid

#13527

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN

Use

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

none

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pCDB326

Plasmid

#113670

Purpose

expresses Ulp1_R2 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R2

Use

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pOX-twk19

Plasmid

#16119

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel twk-19 (unc-58 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pOX-twk16

Plasmid

#16444

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

C.elegans Tandem pore domain K+ channel twk-16 (twk-16 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pBSU6-shTOM40.3

Plasmid

#89796

Purpose

Expresses shRNA against TOM40.

Depositor

Robert Friedlander

Article

Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

Insert

shRNA Tom40

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pOX-twk41

Plasmid

#16127

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (egl-23 Nematode)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

May 13, 2008

Availability

Academic Institutions and Nonprofits only
UbcH10

Plasmid

#8504

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
XE225 mouse Chibby-pCS2+

Plasmid

#16823

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

mouse Chibby-pCS2+ (cby Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pGST1-yGGA2-VHS

Plasmid

#44422

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA2 (1-169) (GGA2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNAOX-twk46

Plasmid

#16129

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Voltage-gated K+ channel (twk-46 Nematode)

Use

Mammalian expression & oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pDONOR 6colors

Plasmid

#229974

Purpose

bigMamAct assembled plasmid for 6colors cellular labelling

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin

Use

CRISPR

Tags

Expression

Mammalian

Mutation

See Depositor Comments

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2837

Plasmid

#231676

Purpose

Expresses a rabbit IgH signal peptide fused to GFP in mammalian cells

Depositor

Damian Ekiert

Article

Jaroenlak et al bioRxiv [Preprint]. 2024 May 30:2024.05.

Insert

Rabbit IgH-sfGFP

Use

Tags

sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL2142 MTK3-sfGFP

Plasmid

#226661

Purpose

MTK3 part containing the coding sequence of sfGFP

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJB75

Plasmid

#226320

Purpose

E. coli protein expression for protein purification

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

6xHis-(Y to A MR1-6)-strep

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

N-terminal domain deleted, C-terminal domain dele…

Promoter

T7

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJB72

Plasmid

#226312

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1

Use

Tags

Expression

Bacterial

Mutation

Y300A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB44

Plasmid

#226303

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with C to S MR1

Use

Tags

Expression

Bacterial

Mutation

C292S, C310S

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB43

Plasmid

#226302

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

WT CsoS2, frameshift site intact

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBEL2957

Plasmid

#222972

Purpose

To express a putative signal peptide from Encephalitozoon intestinalis protein Eint_060150 fused to GFP in mammalian cells

Depositor

Damian Ekiert, Gira Bhabha

Article

Jaroenlak et al bioRxiv 2024.05.30.596468

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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