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Showing: 9751 - 9800 of 148090 results

  1. pTPGI_dCas9_VP64

    Plasmid
    #49013
    Purpose
    encodes yeast-optimized dCas9_VP64 synthetic transcription factor
    Depositor
    Timothy Lu
    Article
    Farzadfard et al ACS Synth Biol. 2013 Sep 11.
    Insert
    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    pTPGI (galactose+aTc inducible)
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49013-plasmid-map-sequence-id-231494

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  5. pBiFC-PBZ-4A-VN

    Plasmid
    #110647
    Purpose
    expresses a PAR-binding (PBZ) domain fused to venus N-terminus, contains point mutations that abolish PAR binding
    Depositor
    Chris Lord
    Article
    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:
    Insert
    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    Use
    Tags
    DDK and VN
    Expression
    Mammalian
    Mutation
    C379A, C385A, C421A and C427A
    Promoter
    CMV
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110647-plasmid-map-sequence-id-217748

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  7. pET-His6-SUMO-MproC145A

    Plasmid
    #172653
    Purpose
    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO
    Depositor
    Stephen Harrison
    Article
    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25
    Insert
    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    6xHis Tag and SUMO
    Expression
    Bacterial
    Mutation
    Catalytic Cysteine 145 mutated to Alanine
    Promoter
    T7
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    172653-plasmid-map-sequence-id-370049

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  8. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-tubulin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

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  13. MIG-Luciferase-IRES-GFP

    Plasmid
    #75021
    Purpose
    Encodes the expression of Luciferase and GFP
    Depositor
    Saradhi Mallampati
    Insert
    Luciferase
    Use
    Luciferase and Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    75021-plasmid-map-sequence-id-337469

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  14. ECFP-Nucleus-7

    Plasmid
    #55346
    Purpose
    Localization: SV40 NLS Triplet, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    ECFP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55346-plasmid-map-sequence-id-182186

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  15. TH mAb_BCN53.1.1D10_HC_Mouse IgG2a

    Plasmid
    #182896
    Purpose
    Mammalian expression of TH mouse mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-TH (human) heavy chain (TH Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182896-plasmid-map-sequence-id-360557

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  18. TGN38 EGFP

    Plasmid
    #128148
    Purpose
    Expression of TGN38 amino acids 304-357
    Depositor
    Jennifer Lippincott-Schwartz
    Insert
    TGN38 (Tgoln2 Rat)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Contains amino acids 304-357
    Promoter
    Available since
    Nov. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128148-plasmid-map-sequence-id-251067

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  28. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Hans Zempel
    Insert
    POLG (POLG Human)
    Use
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    191487-plasmid-map-sequence-id-415364

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  34. pAAV-Ef1a-DIO EYFP

    Plasmid
    #27056
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27056-plasmid-map-sequence-id-126562

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  37. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  38. R777-E005 Hs.AKT3

    Plasmid
    #70289
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    70289-plasmid-map-sequence-id-125738

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  40. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.GPI

    Plasmid
    #196223
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLP-FRT iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196223-plasmid-map-sequence-id-405486

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Showing: 9751 - 9800 of 148090 results