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Plasmid#92139PurposeTargeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selectionDepositorUseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only
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pAB46 (CK2beta)
Plasmid#27085DepositorInsertCK2beta (CSNK2B Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-sfCherry11x4-ß-actin
Plasmid#70220PurposeExpresses sfCherry11x4-ß-actin in mammalian cellsDepositorInsertsfCherry11x4-actin (ACTB Human)
UseTagssfCherry11x4ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry
Plasmid#50458PurposeDouble floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas ServiceAAV8InsertrM3D-mCherry
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2 HRS-RFP
Plasmid#29685DepositorInserthepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)
UseTagsRFPExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBABe-HA-ER-IPpoI
Plasmid#32565DepositorInsertI-PpoI
UseRetroviralTagsER and HAExpressionMammalianMutationnonePromoterAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHREAC
Plasmid#134908PurposeOverexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterdouble 35SAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC18-8XLexAop2-mCD8::GFP
Plasmid#26225DepositorInsertmCD8::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4H5
Plasmid#71716PurposeMembrane scaffold protein (MSP), helix 4+ helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luciferase
Plasmid#21375Purposeself-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and LuciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFLAG-HHARI
Plasmid#17450DepositorInsertHHARI (ARIH1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2008AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-SpHis5
Plasmid#125704PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selectionDepositorInsertymNeongreen
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
LeGO-EBFP2
Plasmid#85213PurposeLentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoterDepositorInsertEBFP2
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA-IRES-Hygro
Plasmid#102423PurposeMammalian expression vector for tetracycline-transactivator (rtTA), IRES-HygroDepositorInserttetracyclin-transactivator (rtTA)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
Plasmid#20942Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsExpressionMammalianMutationH134R mutation in ChR2PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL hygro Flag HA TRAP220 wt
Plasmid#17433PurposeRetroviral expression of Med1/TRAP220 in mammalian cellsDepositorInsertTRAP220 (MED1 Human)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,42a]
Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only