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Plasmid#97024PurposeExpressing Pyrobaculum spherical virus orf165a proteinDepositorInsertPSV orf 165a
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF137
Plasmid#97023PurposeExpressing Pyrobaculum spherical virus orf137 proteinDepositorInsertPSV orf 137
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA1376
Plasmid#97002PurposeExpressing MRSA sar1376 proteinDepositorInsertPutative 4-oxalocrotonate tautomerase
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationnonePromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPSVORF131
Plasmid#97022PurposeExpressing Pyrobaculum spherical virus orf131 proteinDepositorInsertPSV orf 131
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISPA4511
Plasmid#97015PurposeExpressing Pseudomonas aeruginosa orf PA4511 proteinDepositorInsertP. aeruginosa orf PA4511
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSATAG
Plasmid#97004PurposeExpressing MRSA TAG proteinDepositorInsertDNA-3-methyladenine glycosylase I
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationPromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4F2
Plasmid#73431PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4F2.DepositorInsertRepressor 4F2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1B6
Plasmid#73430PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.DepositorInsertRepressor 1B6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3H5
Plasmid#73429PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.DepositorInsertRepressor 3H5 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1D4
Plasmid#73433PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.DepositorInsertRepressor 1D4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-1E4
Plasmid#73436PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.DepositorInsertRepressor 1E4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-5F5
Plasmid#73432PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.DepositorInsertRepressor 5F5 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucA
Plasmid#174047PurposeExpresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinaseDepositorInsertLucA
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationALG8 alanine 506 to threoninePromoterHuman Ubiquitin CAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Lenti-LucL
Plasmid#174048PurposeExpresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinaseDepositorInsertLucL
UseCre/Lox, Lentiviral, and LuciferaseTagsLuciferaseExpressionMammalianMutationLAMA4 glycine 1254 to valinePromoterHuman Ubiquitin CAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107
Plasmid#64615Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-HK2
Plasmid#23854DepositorInsertHK2 (HK2 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRRL-MND-hCard11 WT-T2A-GFP
Plasmid#163787PurposeExpression of Wild-type CARD11DepositorInsertCARD11 (CARD11 Human)
UseLentiviralTagsExpressionMutationPromoterMNDAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-10xHis
Plasmid#194178PurposeExpresses MPP8-10xHis taggedDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1ā110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s(alpha3beta5/G226A/A366S)
Plasmid#55797PurposeThis highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.DepositorInsertalpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
UseTagsinternal EE epitope (residues 189-194 in alpha-s ā¦ExpressionMammalianMutationN271K, K274D, R280K, T284D, I285T, G226A, A366S iā¦PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only