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pLV-hSyn1-GFP

Plasmid

#177810

Purpose

Encodes a GFP driven by human synapsin I promoter

Depositor

Christopher Grigsby

Article

Mattiassi et al Biomater Sci. 2021 Aug 7;9(15):5175-5191

Insert

CopGFP inserted behind human synapsin I promoter (SYN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Syn1

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP 150TAG

Plasmid

#85483

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150TAG (amino acid 150 mutated to stop codon)

Promoter

Arabinose

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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PresentER-SIINFEKL (GFP)

Plasmid

#102944

Purpose

PresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

SIINFEKL (OVAL Synthetic, Chicken)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

OVA257-264

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

hrGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP 150TAG

Plasmid

#197574

Purpose

Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

N150TAG

Promoter

araC and lpp

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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CDH5-AbR-GFP

Plasmid

#122970

Purpose

Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

Depositor

Budd A. Tucker

Inserts

CDH5p
CopGFP
Zeocin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-eDHFR-MCS

Plasmid

#172108

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

Depositor

Shinya Tsukiji

Article

Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

Insert

EGFP-eDHFR-20aa (folA E. coli)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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VCP(R155H)-EGFP

Plasmid

#23972

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

R155H

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).cpSFGFP.HaloTag

Plasmid

#214925

Purpose

Expresses mitochondrially targeted non-responsive control

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-P4Mx1

Plasmid

#108121

Purpose

PI4P biosensor

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

X.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Article

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Use

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7 promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQE-60NA-msGFP2

Plasmid

#135301

Purpose

Encodes a photostable monomeric superfolder GFP

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

msGFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP_Hu_p115-RhoGEF(WT)

Plasmid

#136337

Purpose

Mammalian expression of human p115-RhoGEF with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ARHGEF1 (ARHGEF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHR' CMV GFP

Plasmid

#14858

Depositor

Inder Verma

Article

Miyoshi et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

CMV-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-mEGFP

Plasmid

#186329

Purpose

Fluorescent reporter system

Depositor

Bertrand Collet

Insert

mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP

Plasmid

#46905

Purpose

expresses EGFP tagged Tau AP in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A T22N

Plasmid

#28048

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Use

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

T22N

Promoter

Available since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pEIGW Grx1-roGFP2

Plasmid

#64990

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHR GFP-CaaX

Plasmid

#113020

Purpose

for lentivirus production of GFP-CaaX

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

GFP-CaaX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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