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Plasmid#127307PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)DepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(59)-FFLuc-3XFLAG
Plasmid#127337PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59DepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-W340A.pcDNA3-k
Plasmid#127404PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site.DepositorInsertMaltose Binding Protein
UseTagsFLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon and W34…PromoterCMVAvailable sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RPS14(89-151)
Plasmid#122299Purposeexpresses a part of RPS14 protein with a flag tagDepositorInsertRPS14 (RPS14 Human)
UseTagsFlagExpressionMammalianMutationdeleted amino acids 1 to 88PromoterCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 d835
Plasmid#113959Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-834PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-hTie1-ECD-FLAG
Plasmid#119235PurposePlasmid encodes FLAG-tagged extracellular domain of human Tie1 gene cloned in lentiviral vector.DepositorInsertTIE1-ECD-FLAG (TIE1 Human)
UseLentiviralTagsFLAGExpressionMutationContains extracellular domain (ECD) onlyPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
IRES-EGFP-Flag-hRabin8
Plasmid#118073PurposeExpresses Flag-tagged human Rabin8 in mammalian cellsDepositorInserthuman Rabin8 (RAB3IP Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1 alphaAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FRB-TALETS3-3xFLAG-2xNLS
Plasmid#107306PurposeExpresses TALE-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertTALE_VEGFA-TS3
UseCRISPRTags3x Flag, 2xNLS and FRBExpressionMammalianMutationPromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag RagC (D181N)
Plasmid#112752Purposeexpress Flag-RagC (D181N mutant)DepositorInsertFlag-RagC (D181N) (RRAGC Human)
UseTagsFlagExpressionMammalianMutation181NPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
B9-COMP-blac-flag-his
Plasmid#111020PurposeExpresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.DepositorInsert6-cysteine protein (B9) (PF3D7_0317100 )
UseTagsExogenous signal peptide of mouse origin and rat …ExpressionMammalianMutationAll NXT/S sites mutated to NXA, where X is any am…PromoterCMVAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-mRIPK1-S321A
Plasmid#115339PurposeMammalian expression of Flag-mRIPK1-S321ADepositorInsertRIPK1 (Ripk1 Mouse)
UseTagsFlagExpressionMammalianMutationS321APromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL-FLAG/His LCOR
Plasmid#114450PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human LCOR coding sequenceDepositorInsertLCOR (LCOR Human)
UseTagsFLAG-HisExpressionInsectMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)
Plasmid#112803PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 554_557 deleted/PDZPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
Plasmid#112802PurposeExpression of OCTN2 mutantDepositorInsertOCTN2/SLC22A5 (Slc22a5 Rat)
UseTags3 x FlagExpressionMammalianMutationamino acids 14_22 and 447_454 deletedPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK480] pLJC5 Flag-Depdc5(A)
Plasmid#109338PurposeLenti-viral expression of Flag-tagged Depdc5 mutant ADepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationDIYGD(185-189)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK481] pLJC5 Flag-Depdc5(B)
Plasmid#109339PurposeLenti-viral expression of Flag-tagged Depdc5 mutant BDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationEQPLH(371-375)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
[pSK479] pLJC5 Flag-Depdc5(C)
Plasmid#109340PurposeLenti-viral expression of Flag-tagged Depdc5 mutant CDepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFLAGExpressionMutationFTPRI(419-423)GSGSGPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A
Plasmid#109031PurposeD16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-N3FLAG-TAF12
Plasmid#105593Purposeretrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistanceDepositorInsertfull length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
UseRetroviralTags3*FLAGExpressionMammalianMutationsilent mutations to make it resistant to the targ…PromoterMSCV-LTRAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
MDH1-PGK-FLAG-TAT-86-D
Plasmid#35976DepositorInserttransactivating protein (tat Human Immunodeficiency Virus)
UseRNAi and RetroviralTagsFlagExpressionMammalianMutationFrom the "D" HIV cladePromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only