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Plasmid#164503PurposeTo generate a stable acceptor cell line with a BxbI attP site at the AASV1 locus, between an exogenous human pRPL30 promoter and a tripartite V5/SNAPtag/Blast reporter.DepositorInsertV5-SNAPtag-PDGFR_TM-T2A-Blast
UseTo generate attp acceptor cell lines for bxbiTagsExpressionMammalianMutationPromoterhuman RPL30Available sinceAvailabilityAcademic Institutions and Nonprofits only -
53 pLIB_MBP
Plasmid#160301PurposeN-term 3C cleavable MBP tag for insect cell expressionDepositorTypeEmpty backboneUseTags6xHis and MBPExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
50 pLIB_STREP
Plasmid#160275PurposeN-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB33eCPX
Plasmid#23336DepositorInsertEnhanced Circularly Permuted Outer Membrane Protein X
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
GabaARD-GFP/pCDNA
Plasmid#197321PurposePlasmid expressing an antigen targeting the GABA-A Receptor deltaDepositorInsertGABA A delta (GABRD Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD
Plasmid#32055PurposeVector optimized for inducible gene expression in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPbadAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
p406CYC1
Plasmid#15975DepositorInsertCYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationCYC1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iPASnFR-L
Plasmid#225238PurposeBacterial expression of iPASnFR-LDepositorInsertiPASnFR-L
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GABA BR2-flag/pRK5
Plasmid#197323PurposePlasmid expressing an antigen targeting the GABA(B)R2 metabotropic GABA receptorDepositorInsertGABA B R2 (Gabbr2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mg319
Plasmid#73367Purposeunc-70 tension sensorDepositorInsertunc-70::TSMod
UseTagsExpressionMutationPromoterunc-70pAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-iPASnFR-H
Plasmid#225237PurposeBacterial expression of iPASnFR-HDepositorInsertiPASnFR-H
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mT-Sapphire-C1
Plasmid#54545PurposeLocalization: C1 Cloning Vector, Excitation: 399, Emission: 511DepositorTypeEmpty backboneUseTagsmT-SapphireExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
nGCaMP2-NpuDnaEn
Plasmid#34850DepositorInsertnGCaMP2-NpuDnaEn
UseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7 lacAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
UseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7 lacAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAP1-3
Plasmid#71258Purposeluciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoterDepositorInsert3 copies of an AP-1 site from the human Stromelysin-1 gene (MMP3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterGM-CSF minimalAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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8 pCOLI_A_GST
Plasmid#160150PurposeN-term GST 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LRP4-Flag/pCDNA3.1
Plasmid#197291PurposePlasmid expressing an antigen targeting LRP4 (extracellular)DepositorInsertLRP4 (Lrp4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-hChibby1
Plasmid#89472PurposeLabels mother centrioles/basal bodiesDepositorInserthuman Chibby1 (CBY1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
28 pCOLI_S_STREP
Plasmid#160262PurposeN-term 2xStrepII 3C cleavable Spec resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastbacHTB HA-human PP2A Cα D88N
Plasmid#132633Purposeexpresses HA-human PP2A Cα D88N in Hi5 insect cellDepositorInsertPP2A Cα D88N
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD-eGFP
Plasmid#112918PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPbadAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
IMBB2.4-pUC57-mini
Plasmid#88845Purposeplasmid for "Tip Snip" cloning (described in forthcoming Biotechniques article)DepositorTypeEmpty backboneUseSynthetic Biology; CloningTagsExpressionMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mGlurR5a/pCI-Neo
Plasmid#197305PurposePlasmid expressing an antigen targeting the mGluR1/5 metabotropic glutamate receptorDepositorInsertmGluR5a (Grm5 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
19 pCOLI_A_SUMO
Plasmid#160257PurposeN-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
56 pLIB_CBP
Plasmid#160305PurposeN-term 3C cleavable CBP tag for insect cell expressionDepositorTypeEmpty backboneUseTagsCBPExpressionInsectMutationPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
9 pCOLI_A_C_GST
Plasmid#160245PurposeC-term GST uncleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTagsGSTExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSlo2.1 Slick/pCDNA3
Plasmid#197317PurposePlasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunitDepositorInsertSlo2.1 (Kcnt2 Mouse)
UseTags7xHisExpressionMammalianMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVU-tTR-KRAB
Plasmid#11645DepositorInserthUbiquitin C, GFP, tTR-KRAB, no tetO
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
UVR8-mCherry
Plasmid#49804Purposeexpresses mCherry fused to UVR8 under the CMV promoterDepositorInsertUVR8-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p404CYC1
Plasmid#15971DepositorInsertCYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationCYC1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
40 pCOLI_K_MBP
Plasmid#160183PurposeN-term MBP 3C cleavable Kan resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
17 pCOLI_A_SNAP
Plasmid#160254PurposeN-term His-SNAP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and SNAPExpressionBacterialMutationPromoterT7Available sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
18 pCOLI_A_Trx
Plasmid#160255PurposeN-term His-Thioredoxin 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and ThioredoxinExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2042.2
Plasmid#140670Purpose3-color stripeDepositorInsert3-color stripe
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
11 pCOLI_A_MBP
Plasmid#160247PurposeN-term MBP 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags6xHis and MBPExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
5 pCOLI_A_STREP
Plasmid#160147PurposeN-term 2xStrepII 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneUseTags2xStrepIIExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only