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Showing: 7761 - 7800 of 148587 results

  5. CXCR2-Tango

    Plasmid
    #66260
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CXCR2 (CXCR2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66260-plasmid-map-sequence-id-344624

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  8. pSm-Nuclease

    Plasmid
    #132431
    Purpose
    Expresses Serratia marcescens nuclease in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    Serratia marcescens nuclease (codon optimized)
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    132431-plasmid-map-sequence-id-258770

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  10. pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

    Plasmid
    #188375
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188375-plasmid-map-sequence-id-371126

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  11. EcSTH

    Plasmid
    #211923
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.
    Depositor
    Binghui Li
    Article
    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211923-plasmid-map-sequence-id-429006

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  17. pCMV-2E2-HA-mEGFP

    Plasmid
    #129595
    Purpose
    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129595-plasmid-map-sequence-id-254267

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  21. pLEX305_3xFLAG_STAT1

    Plasmid
    #220323
    Purpose
    Expresses full length human STAT1 with an N-terminal 3xFLAG tag
    Depositor
    Jonathan Brown
    Insert
    STAT1 alpha (STAT1 Human)
    Use
    Lentiviral
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    PGK
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220323-plasmid-map-sequence-id-437225

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  22. pET29b-H6_Streptavidin_sfGFP

    Plasmid
    #124296
    Purpose
    His6-tagged Streptavidin GFP fusion protein
    Depositor
    Mark Arbing
    Insert
    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    Use
    Tags
    His6 and T7 tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    124296-plasmid-map-sequence-id-240453

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  23. pDONR221-SLC3A2_STOP

    Plasmid
    #161379
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC3A2 (SLC3A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161379-plasmid-map-sequence-id-349390

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  25. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  30. pDisplay-NE2h-IRES-mCherry-CAAX

    Plasmid
    #208691
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells
    Depositor
    Yulong Li
    Insert
    GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208691-plasmid-map-sequence-id-410345

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  32. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  33. pT18mobsacB

    Plasmid
    #72648
    Purpose
    sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72648-plasmid-map-sequence-id-399780

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  34. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja1 (Gja1 Rat)
    Use
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69007-plasmid-map-sequence-id-121423

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  35. pSNAPf-N1

    Plasmid
    #58187
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

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Showing: 7761 - 7800 of 148587 results