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pPOTv7-blast-mNG:Ppyr9eh

Plasmid

#201441

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mNG:Ppyr9eh

Use

Unspecified

Tags

Expression

Mutation

Promoter

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Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mScarletI:His

Plasmid

#201098

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

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Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-His:mScarletI:His

Plasmid

#201097

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mScarlet-I

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-g418-His:mNG:His

Plasmid

#201095

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mNeonGreen

Use

Unspecified

Tags

Expression

Mutation

Promoter

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Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-tagRFPt

Plasmid

#201085

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

tagRFPt

Use

Unspecified

Tags

Expression

Mutation

Promoter

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Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-tagRFPt

Plasmid

#201084

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

tagRFPt

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-eYFP

Plasmid

#190219

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

eYFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mGL

Plasmid

#190221

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mGreenLantern

Use

Unspecified

Tags

Expression

Mutation

Promoter

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Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6.1-phleo-Ty:mNG:Ty

Plasmid

#181936

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

mNG

Use

Unspecified

Tags

Expression

Mutation

Promoter

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Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10V5

Plasmid

#181938

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

10xV5

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-HaloTag

Plasmid

#181953

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al bioRxiv 2023.04.21.537815

Insert

HaloTag

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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T_antitdTomatosynsrRNA_UA

Plasmid

#185975

Purpose

Expresses antitdTomatosynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

PlTetO-1 AntitdTomatosynsrRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PlTetO-1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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T_antiFtszsynsrRNA_UA

Plasmid

#185976

Purpose

Expresses antiFtszsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

PlTetO-1 AntiFtszsynsrRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PlTetO-1

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pICH84011

Plasmid

#48035

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pICH83988

Plasmid

#48032

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pICH83999

Plasmid

#48033

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pICH83955

Plasmid

#48036

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-CybGam-WPRE

Plasmid

#207662

Purpose

Control for CybSEP2

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

Gamillus

Use

AAV

Tags

Expression

Mutation

Histidine 86 and 159 to alanines, inserted gamill…

Promoter

hSynapsin

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pX330A-FBL/PITCh

Plasmid

#63671

Purpose

Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only
pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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