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BUB1B A8.1 gRNA

Plasmid

#90555

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB1B (Guide Designation A8.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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AURKB A4.1 gRNA

Plasmid

#90545

Purpose

3rd generation lentiviral gRNA plasmid targeting human AURKB

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

AURKB (Guide Designation A4.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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BUB1 A5.1 gRNA

Plasmid

#90552

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB1 (Guide Designation A5.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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BUB1 A6.1 gRNA

Plasmid

#90553

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB1 (Guide Designation A6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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CCNB1 B4.1 gRNA

Plasmid

#90577

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNB1 (Guide Designation B4.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CCNB1 B3.1 gRNA

Plasmid

#90576

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNB1 (Guide Designation B3.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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DBF4 C9.1 gRNA

Plasmid

#90646

Purpose

3rd generation lentiviral gRNA plasmid targeting human DBF4

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DBF4 (Guide Designation C9.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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DSN1 C11.1 gRNA

Plasmid

#90656

Purpose

3rd generation lentiviral gRNA plasmid targeting human DSN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DSN1 (Guide Designation C11.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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DSN1 C12.1 gRNA

Plasmid

#90657

Purpose

3rd generation lentiviral gRNA plasmid targeting human DSN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DSN1 (Guide Designation C12.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CENPF C6.1 gRNA

Plasmid

#90617

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPF (Guide Designation C6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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ESPL1 D7.1 gRNA

Plasmid

#90682

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESPL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESPL1 (Guide Designation D7.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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GINS1 D11.1 gRNA

Plasmid

#90694

Purpose

3rd generation lentiviral gRNA plasmid targeting human GINS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

GINS1 (Guide Designation D11.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-A

Plasmid

#85216

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-B

Plasmid

#85217

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-B

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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GTU-c

Plasmid

#81093

Purpose

Gene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

yemCFP

Use

Cre/Lox

Tags

yemCFP

Expression

Yeast

Mutation

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GTU-y

Plasmid

#81095

Purpose

Gene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCGS652

Plasmid

#62220

Purpose

Mammalian expression TALEN vector with N152, C+18 truncations

Depositor

Daniel Voytas

Article

Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

Type

Empty backbone

Use

TALEN

Tags

AcV5 and SV40 NLS

Expression

Mammalian

Mutation

Promoter

miniCaggs

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-Spec(x2)-DocI

Plasmid

#58713

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Spec(x2)-DocI

Use

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Mc2r

Plasmid

#48174

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Tas2r3-mod

Plasmid

#48175

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> ACG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

TAS2R3 taste 2 receptor member 3 (TAS2R3 Human)

Use

Luciferase

Tags

Expression

Mutation

T/C MUTATION IN ATG OF UORF

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only