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PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

Plasmid

#92402

Purpose

Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Kalyviotis et al (2020) Behavioral and Neural Genetics of

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and No tag, cytosolic expression

Expression

Mutation

Promoter

bactin2

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFPmem pA

Plasmid

#80823

Purpose

CMVmin-promoted GFPmem with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

3E-CMV-GFPCAAXpA spacer

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Hox A11a

Plasmid

#36462

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11a (hoxa11a Zebrafish)

Use

Riboprobe synthesis

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pFUS_A3A

Plasmid

#43851

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a-CylA

Plasmid

#48198

Purpose

Expresses CylA (fatty acyl-AMP ligase) in E. coli

Depositor

Emily Balskus

Article

Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

Insert

CylA

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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T7-WbpB-His6

Plasmid

#89711

Purpose

Expresses WbpB from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

WbpB (wbpB Pseudomonas aeruginosa)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-WbpE-His6

Plasmid

#89712

Purpose

Expresses WbpE from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

WbpE (wbpE Pseudomonas aeruginosa)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-WbpD-His6

Plasmid

#89713

Purpose

Expresses WbpD from P. aeruginosa with an N-terminal T7 tag and a C-terminal His6 tag

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

WbpD (wbpD Pseudomonas aeruginosa)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)tdimer2W

Plasmid

#27233

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

DsRed2

Use

Lentiviral

Tags

tandem dimer RFP

Expression

Mutation

Promoter

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGEMHEZFCNGA5

Plasmid

#69861

Purpose

oocyte expression vector containing ZFCNGA5

Depositor

Gary Matthews

Article

Tetreault et al Biochem Biophys Res Commun. 2006 Sep 22;

Insert

ZFCNGA5 (cnga5 Zebrafish)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

none

Promoter

T7

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pSL10 - Brr2 del422 WT

Plasmid

#90260

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Use

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLSLT

Plasmid

#51498

Purpose

pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

T30 TALEN pair
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pFUS_A4B

Plasmid

#43854

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-luxAB2

Plasmid

#27065

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB integrative

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pchfn01

Plasmid

#14061

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
p35SZ

Plasmid

#51513

Purpose

pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant expression t-dna

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)-DR RecR(34-220)

Plasmid

#12618

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR(34-220)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-33

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pTE-0X38-GCT6

Plasmid

#27057

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-gfp-DAS+4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pLPE1

Plasmid

#209981

Purpose

Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synthetic Biology, 2024

Insert

E. coli shuttle part (pMB1_ampR_B0017)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLPE2

Plasmid

#209982

Purpose

Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synthetic Biology, 2024

Insert

E. coli shuttle part (ampR_B0017)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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