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pOME0135

Plasmid

#178397

Purpose

KSHV Kaposincluster-RevCompl-Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Kaposincluster-RevCompl-SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0134

Plasmid

#178396

Purpose

Expresses KSHV Upstream Mir-SYN

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV Upstream Mir SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0133

Plasmid

#178394

Purpose

Expresses KSHV Downstream Mir-SYN

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV Downstream Mir-SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0235M

Plasmid

#178417

Purpose

KSHV Reverse compl Sequence Kaposincluster_RevCompl-KapATGdel_Syn

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

ReverseComplement-Kaposincluster_RevCompl_KapATGdel_Syn

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

Plasmid

#155308

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfAMA1

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

K546M/I547P/I548Y/I549Y

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-PfROM4 (G470A/G471N)

Plasmid

#154859

Purpose

Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM4 (PF3D7_0506900 Synthetic)

Use

Tags

triple HA

Expression

Mammalian

Mutation

Glycine 470 changed to Alanine and Glycine 471 ch…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-PfROM1 (A144G/N145G)

Plasmid

#154873

Purpose

Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfROM1 (PF3D7_0506900 Synthetic)

Use

Tags

triple HA

Expression

Mammalian

Mutation

Alanine 144 changed to Glycine and Asparagine 145…

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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miniABEMax V82G wt

Plasmid

#135362

Purpose

Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-GPR78

Plasmid

#106033

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR78; Opto-GPR78; C11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR78 (GPR78 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR42

Plasmid

#106022

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR42; Opto-GPR42; B12)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR42 (GPR42 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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hHSF4b K294R

Plasmid

#118357

Purpose

This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Use

Tags

Expression

Mammalian

Mutation

K294R

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3_altPKA-Cα-mEGFP

Plasmid

#114132

Purpose

Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

18747 (Prkaca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 N181-211 (#595)

Plasmid

#99939

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA181-211insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 N187-207 (#596)

Plasmid

#99940

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

delAA187-207insNAAIRS linker

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 85-232 (#427)

Plasmid

#99922

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 85-232

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 101-232 (#183)

Plasmid

#99923

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N-terminal deletion; encodes AA 101-232

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 1-192 (#421)

Plasmid

#99925

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-192

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 1-209 (#430)

Plasmid

#99926

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal deletion; encodes AA 1-209

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 96-110 (#189)

Plasmid

#99929

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 96-110

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-REDD1 111-135 (#190)

Plasmid

#99930

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Internal deletion of AA 111-135

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge