Addgene

Showing: 3841 - 3860 of 37253 results

Results per page:

Sort results by:

Dscam1_7.27.25 EC10-AP-His

Plasmid

#72062

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA

Plasmid

#108785

Purpose

Expresses N-terminus to aa269 of VCre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment N-terminus to aa269

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA

Plasmid

#108786

Purpose

Expresses aa270 to C-terminus of VCre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa270 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-FLAG-TRAF4(K190R)

Plasmid

#58317

Purpose

FLAG-tagged mouse TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pMAL c2x Tral FL

Plasmid

#113012

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral WT

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS1978

Plasmid

#49110

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

C8ORF46

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBacPak-HA-Non-stop

Plasmid

#52289

Purpose

Expresses Drosophila Non-stop

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Non-stop (not Fly)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
pEMS2026

Plasmid

#49133

Purpose

AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple301-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TNNT1-B

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS1988

Plasmid

#49119

Purpose

AAV plasmid with CAGGS promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T35A

Plasmid

#47937

Purpose

expresses Myc tagged human MOB1 containing T35A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
3605 pGEX4T-1 Bad S112A S136A (104-141)

Plasmid

#8803

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S112A, S136A

Promoter

Ptac

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pPSt_35Spr-GFP-t35S

Plasmid

#203592

Purpose

Fully assembled Plant STARR-seq plasmid with the 35S terminator.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Inserts

CaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8709

Plasmid

#175224

Purpose

Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YOL092W (CEN.PK strain)

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Expression

Mutation

Promoter

T7

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti hSyn HK1

Plasmid

#220915

Purpose

Neuronal specific expression of HK1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

HK1 (HK1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Bn CAAX pcDNA3

Plasmid

#206088

Purpose

Cav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge