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Plasmid#163003Purposeplasmid expressing evoABEDepositorInsertevoABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9784-xABE
Plasmid#163005Purposeplasmid expressing xABEDepositorInsertxABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T2U2T2-YFP
Plasmid#84550PurposeHybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFPDepositorInsertU2T2U2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U2T2U2-YFP
Plasmid#84552PurposeHybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFPDepositorInsertT2U2T2U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher
Plasmid#157674PurposeExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertSpyCatcher with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod(XL2V)-Doc
Plasmid#157676PurposeExpression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.DepositorInsertXMod-Doc mutant XL2V with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-no charge
Plasmid#160532PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-no charge
UseTagsHis-TagExpressionBacterialMutationY208C, D322C, E337A, N338A, K341A, N342APromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-no charge
Plasmid#160525PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-no charge
UseTagsHis-TagExpressionBacterialMutationA221C, N404C, E337A, N338A, K341A, N342APromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNAflaB
Plasmid#149561PurposeflaB gRNA expression in B. burgdorferiDepositorInsertsgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNArodA
Plasmid#149569PurposerodA gRNA expression in B. burgdorferiDepositorInsertsgRNArodA
UseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
R2x2_VAL88
Plasmid#160761PurposeExpresses R2x2_VAL88 in bacterial cellsDepositorInsertR2x2_VAL88
UseTags6xHisExpressionBacterialMutation10-residue mutations of Rsmn2x2_5_6PromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_P0826S-sgRNA500
Plasmid#149618PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterP0826SAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PresTL-sgRNA500
Plasmid#149617PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPresTLAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXW101Plux2-Pecf11-gfp
Plasmid#160823PurposeTwo-layered AHL-responsive circuit carrying an ECF11-based amplifierDepositorInsertPJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPJ23101, Plux2, Pecf11Available sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBSV2H_Psyn-sgRNA500
Plasmid#149559PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (D3 - L39V)
Plasmid#136607PurposeExpresses DANCER-3 mutant (reversion L39V)DepositorInsertDANCER-3 mutant (L39V reversion)
UseTags5x-HistagExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V54IPromoterT7 promoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (D3 - I54V)
Plasmid#136608PurposeExpresses DANCER-3 mutant (reversion I54V)DepositorInsertDANCER-3 mutant (I54V reversion)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-A34F-V39LPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-11a (D3 - F34A)
Plasmid#136606PurposeExpresses DANCER-3 mutant (reversion F34A)DepositorInsertDANCER-3 mutant (F34A reversion)
UseTags6x-HistagExpressionBacterialMutationMutation from GB1 WT: Y3F-L7I-V39L-V54IPromoterT7 promoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only