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Plasmid#118368PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (HA-GFP1-10) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsHA-GFP1-10ExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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mCherry-Peroxisomes-2
Plasmid#54520PurposeLocalization: Peroxisomes, Excitation: 587, Emission: 610DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.4/5 Spike
Plasmid#186031PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4/5 SpikeDepositorInsertSARS-CoV-2 BA.4/5 Spike (S )
UseTagsExpressionMammalianMutationBA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBUE411
Plasmid#62200PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3Ă—FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
phND2-N174
Plasmid#31822DepositorInsertNeuroD2 (NEUROD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEN-luxCDABE
Plasmid#44918DepositorInsertsigma 70 promoter
UseLuciferaseTagslux transcriptional fusionExpressionMutationPromoterem7Available sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Antares2 c-myc
Plasmid#100027PurposeA BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescenceDepositorInsertAntares2
UseTagsc-mycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
PT2/C-Luc//PGK-SB13
Plasmid#20207DepositorInsertFlirefly luciferase/Sb transposase
UseTransposonTagsExpressionMutationPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT525
Plasmid#131392PurposeL1RP retrotransposition reporterDepositorInsertL1RP GFP-AI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic EGFP
Plasmid#128053PurposeEGFP reporter vector with restriction sites to clone in a promoter of interest, derived from pGL3 basicDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Fis1
Plasmid#182580PurposeExpresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequenceDepositorInsertmCherry-Fis1
UseTagsFis1ExpressionMammalianMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro_GCaMP6s
Plasmid#164589PurposeStable integration and expression of GCaMP6s in mammalian cellsDepositorInsertGCaMP6s
UseLentiviralTags6xHis, T7 tag, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY31
Plasmid#84746PurposeExpresses huLbCpf1-P2A-puro and crRNA guideDepositorInsertshuLbCpf1
puromycin resistance gene
UseCRISPRTags3xHA, NLS, and T2AExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLIK sh human Rb 1534 hyg
Plasmid#31500DepositorInsertRB shRNA (RB1 Human)
UseLentiviral and RNAiTagsEGFPExpressionMammalianMutationThe target sequence for shRB 1534 is GAACGATTATCC…PromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 flag - ULK2 wt
Plasmid#27637DepositorInsertmouse ULK2 (Ulk2 Mouse)
UseTagsflagExpressionMammalianMutationInitial M removed from ULK2.PromoterAvailable sinceMarch 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 CS puroR
Plasmid#46754PurposeRetroviral vector that expresses catalytically inactive form of HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationC223S--catalytically inactivePromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only