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Addgene
Showing: 3841 - 3860 of 16007 results
  1. pcDNA3.1 Flag YFP hNKCC1 C543M (NT360)

    Plasmid
    #49068
    Purpose
    Expresses human NKCC1 C543M mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C543M in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1 Flag YFP hNKCC1 C630A (NT399)

    Plasmid
    #49071
    Purpose
    Expresses human NKCC1 C630A mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C630A in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3.1 Flag YFP hNKCC1 C630S (NT400)

    Plasmid
    #49072
    Purpose
    Expresses human NKCC1 C630S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C630S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)

    Plasmid
    #49073
    Purpose
    Expresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C723S,C724V in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3.1 Flag YFP hNKCC1 C791S (NT572)

    Plasmid
    #49074
    Purpose
    Expresses human NKCC1 C791S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C791S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3.1 Flag YFP hNKCC1 C820S (NT573)

    Plasmid
    #49075
    Purpose
    Expresses human NKCC1 C820S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C820S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  7. pcDNA3.1 Flag YFP hNKCC1 C1052S (NT674)

    Plasmid
    #49076
    Purpose
    Expresses human NKCC1 C1052S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C1052S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  8. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

    Plasmid
    #49077
    Purpose
    Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x HA and mCerulean
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3.1 Flag YFP hNKCC1 (NT17)

    Plasmid
    #49085
    Purpose
    Expresses human NKCC1 with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    262 silent mutations from native hNKCC1
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  10. GFP(N-glyc)-Tac-TC

    Plasmid
    #162496
    Purpose
    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.
    Depositor
    Insert
    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its TMD and cysotol…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

    Plasmid
    #192445
    Purpose
    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.
    Depositor
    Insert
    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  12. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid
    #49069
    Purpose
    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C563S,C568S,C577S,C582S in hNKCC1 in NT17
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid
    #49070
    Purpose
    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    ECL4 deletion of amino acids 562-582 in hNKCC1 in…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  14. pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)

    Plasmid
    #49078
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  15. pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)

    Plasmid
    #49079
    Purpose
    Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    C295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 3841 - 3860 of 16007 results