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Plasmid#158630PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVS mutantDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationQ148APromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3x-FLAG-CMV-MSTN
Plasmid#157953Purposeexpression of MSTN geneDepositorInsertMSTN (MSTN Sus scrofa)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-388 FLAG miRFP670
Plasmid#130137PurposePPI (BiFC/TriFC) assay vectorDepositorInsertmiRFP670
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-382 FLAG Venus
Plasmid#130133PurposePPI (BiFC/TriFC) assay vectorDepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-720aa)
Plasmid#139687PurposeExpresses N-teminal Flag tagged aa 1- 720 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA8-Flag-LARS(1-976aa)
Plasmid#139689PurposeExpresses N-teminal Flag tagged aa 1-976 of LARS1DepositorInsertLARS1 (LARS1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-GK (281-473)
Plasmid#134273PurposeLentivector encoding Flag-tagged GK truncation (amino acids 281-473)DepositorInsertGk (Gk Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk amino acids 281-473PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE-tight2-Tmem98-FLAG
Plasmid#124506PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsFlagExpressionMammalianMutationPromotertetO-CMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX-FLAG-CH3 Del33
Plasmid#133724PurposeRetroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlagExpressionMutationpartial (not complete) p300 fragment, AT and CH3 …PromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (25-287)
Plasmid#134263PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 25-287)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 25-287PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-C-Flag-GK5 (1-287)
Plasmid#134262PurposeLentivector encoding Flag-tagged GK5 truncation (amino acids 1-287)DepositorInsertGk5 (Gk5 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationmouse Gk5 amino acids 1-287PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-Flag-Snrnp40-skywarp
Plasmid#134250PurposeLentivector encoding Flag-tagged skywarp form of Snrnp40 (missing exon 5)DepositorInsertSnrp40 (Snrnp40 Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationdeleted amino acids 179-219PromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-385 FLAG IFP1.4
Plasmid#130135PurposePPI (BiFC/TriFC) assay vectorDepositorInsertIFP1.4
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-hcRed_IDG-K
Plasmid#135274PurposeGateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-hcRed
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 allKR
Plasmid#129298PurposeExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged all 22 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K295R
Plasmid#129302PurposeExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 295 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
ORF18(R158A_L159A)-3xFLAG pCDNA4
Plasmid#129814PurposeExpresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cellsDepositorInsertORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))
UseTags3xFLAGExpressionMammalianMutationchanged Arginine 158 to Alanine and Leucine 159 t…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ad-mMKP3 S159A S197A-Flag
Plasmid#131579PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsFlagExpressionMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-zeo-ORF24-3xFLAG
Plasmid#130959PurposeLentiviral vector for expression of KSHV ORF24 with a C-terminal 3xFLAG tagDepositorInsertORF24 (ORF24 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseLentiviralTags3xFLAGExpressionMutationPromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only