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pAG175 AK024929 3'UTR mut

Plasmid

#12033

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N8 3'UTR mut (LINC00341 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pRLCon850-1207

Plasmid

#31447

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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p-miR-Report + MT miR-181 BS in NLK 3UTR

Plasmid

#22791

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pAG77 GLCCI1 3'UTR mut

Plasmid

#12062

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

GLCCI1 3'UTR mut (GLCCI1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
CIP2Aprom27bp-pGL4.10Luc

Plasmid

#60876

Purpose

Luc reporter driven by 27 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Tags

Expression

Mutation

Promoter

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pRLCon1-449

Plasmid

#31452

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon1-249

Plasmid

#31453

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLCon1-249_SNP

Plasmid

#31454

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR fragment (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

SNP rs11574744 in miR-34a binding site

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pAG227 CORO1C 3'UTR mut

Plasmid

#12030

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

CORO1C 3'UTR mut (CORO1C Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG83 LRRK1 3'UTR mut

Plasmid

#12058

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N4 3'UTR mut (LRRK1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Cyr61-rDMR

Plasmid

#235692

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Cyr61 (Ccn1 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Cyr61-rDMRdeltaTEAD

Plasmid

#235693

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Cyr61 (Ccn1 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Ctgf-rDMR

Plasmid

#235696

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Ctgf with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Ctgf (Ccn2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Ctgf-rDMRdeltaTEAD

Plasmid

#235697

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Ctgf lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Ctgf (Ccn2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3_5UTR_Myc_FLuc RBM42 deletion

Plasmid

#229503

Purpose

Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_Del 363

Use

Tags

Expression

Mammalian

Mutation

Deletion of bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3_5UTR_Myc_FLuc Compensatory Mutant

Plasmid

#229504

Purpose

Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_CompMutant

Use

Tags

Expression

Mammalian

Mutation

Mutations in bp 363-394

Promoter

SV40

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK-1-miR-455-WT

Plasmid

#215881

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-455-WT (MIR455 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-376a1(3p)-I-type

Plasmid

#215883

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-376a1(3p)-I-type (MIR376A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-589(5p)-I-type

Plasmid

#215884

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(5p)-I-type (MIR589 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-1-miR-589(3p)-I-type

Plasmid

#215885

Purpose

This plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.

Depositor

Kumiko Ui-Tei

Article

Tian et al RNA Biol. 2020 Feb;17(2):264-280. doi: 1

Insert

three tandem repeats of seed-matched (SM) sequence of miR-589(3p)-I-type (MIR589 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge