Addgene

We narrowed to 11,759 results for: CAR

Showing: 3821 - 3840 of 11759 results

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miniCMV-(mNeonGreen)4-tDeg

Plasmid

#129402

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDF850

Plasmid

#228713

Purpose

Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

epPsaCas12f

Use

CRISPR

Tags

Expression

Mammalian

Mutation

I178A/K333V/K454P

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMEH365 Hspa8 HA

Plasmid

#168219

Purpose

Mammalian expression of heat shock 70 kDa protein 8 fused to a C-terminal HA tag

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

Hspa8

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Use

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET24b-APEX2

Plasmid

#111702

Purpose

For IPTG-inducible expression of APEX2 in E. coli

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Use

Tags

6xHis and V5

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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T4 td upstream intron-PABP

Plasmid

#189972

Purpose

Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

T4 td upstream intron-PABP

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSLTS

Plasmid

#59386

Purpose

Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Type

Empty backbone

Use

Genome engineering

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Lac

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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UbC-(mNeonGreen)4-tDeg

Plasmid

#129404

Purpose

(mNeonGreen)4-tDeg fluorogenic protein

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

(mNeonGreen)4-tDeg

Use

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-b

Plasmid

#17591

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-b (SPP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–175, 217–942; See Depositor Comments

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-FlpO

Plasmid

#89574

Purpose

Expresses FlpO under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCBFD1/HBFD1Bad

Plasmid

#53364

Purpose

Will convert beta-carotene into astaxanthin in E. coli when expression of the hbfd1 cDNA is induced with arabinose. Use with pAC-BETA or pAC-BETAipi.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd1

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHBFD1Bad

Plasmid

#53564

Purpose

Produces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

hbfd1 cDNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJF143.J3

Plasmid

#153028

Purpose

J3-mRFP1

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

J3-mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Use

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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