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pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

Plasmid

#105043

Purpose

Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Stk11 (Stk11 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Zfp219-g1)-PGKpuroBFP-W

Plasmid

#105021

Purpose

Lentiviral gRNA plasmid targeting mouse Zfp219 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Zfp219 (Zfp219 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Ndufa2-g1)-PGKpuroBFP-W

Plasmid

#105030

Purpose

Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ndufa2 (Ndufa2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

Plasmid

#105031

Purpose

Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Ndufa2 (Ndufa2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Vps25-g1)-PGKpuroBFP-W

Plasmid

#105033

Purpose

Lentiviral gRNA plasmid targeting mouse Vps25 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Vps25 (Vps25 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(Tcf7l1-g2)-PGKpuroBFP-W

Plasmid

#105016

Purpose

Lentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tcf7l1 (Tcf7l1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304 PCSK5 (R511C)-V5 blast

Plasmid

#83104

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Arginine 511 to Cysteine

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xFLAG SMAD4

Plasmid

#83094

Purpose

Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

SMAD4 (SMAD4 Human)

Use

Entry vector for gateway cloning

Tags

3xFLAG

Expression

Mutation

Promoter

TRE tight promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (T288P)-V5 blast

Plasmid

#83101

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (A270T)-V5 blast

Plasmid

#83102

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Alanine 270 to Threonine

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (H516P)-V5 blast

Plasmid

#83103

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Histidine 516 to Proline

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCIG1_UL136_19kDa_EE

Plasmid

#74951

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

Glu-Glu tag (EYMPME)

Expression

Mammalian

Mutation

delta 1-127

Promoter

CMV, lentiviral LTR

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCIG1_UL136_23kDa_Myc

Plasmid

#74949

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

HA Tag (YPYDVPDYA)

Expression

Mammalian

Mutation

delta 1-99 M128A

Promoter

CMV, Lentiviral LTR

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5 (T288P)-V5 puro

Plasmid

#98709

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Promoter

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIG1_UL136_25kDa_Myc

Plasmid

#74954

Purpose

Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.

Depositor

Felicia Goodrum

Article

Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

Insert

UL136 (UL136 Human Cytomegalovirus strain TB40/E)

Use

Lentiviral

Tags

3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)

Expression

Mammalian

Mutation

delta 1-77, M100A M128A

Promoter

CMV, Lentiviral LTR

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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EYFP-hDF-deltaTM-pCMV-9

Plasmid

#72586

Purpose

human dysferlin no transmembrane domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

transmembrane domain deleted

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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FUP95Q15ADSKGW (D7)

Plasmid

#74030

Purpose

lentiviral expression of Psd95-Q15A deltaSK-EGFP fusion

Depositor

Oliver Schluter, Weifeng Xu, Robert Malenka

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Q15A, delta SH3-GK (deletion of the fragment betw…

Promoter

hUbC

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
KHBD00646

Plasmid

#39708

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1705 (Met Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00543

Plasmid

#39638

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11194 (Hey Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00424

Plasmid

#34530

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6627 (Dnz1 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only