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Showing: 9551 - 9600 of 148090 results

  1. CMV-B-GECO1

    Plasmid
    #32448
    Purpose
    Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    B-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…
    Promoter
    CMV
    Available since
    Sept. 30, 2011
    Availability
    Academic Institutions and Nonprofits only
    32448-plasmid-map-sequence-id-122739

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  7. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid
    #188902
    Purpose
    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9-KRAB(Kox1)-MeCP2
    Use
    Lentiviral
    Tags
    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Sept. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188902-plasmid-map-sequence-id-372712

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  9. pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W

    Plasmid
    #51013
    Purpose
    Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP-2A-mito-RFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51013-plasmid-map-sequence-id-77778

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  15. pT3-EF1aH

    Plasmid
    #180149
    Purpose
    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.
    Depositor
    Xin Chen
    Article
    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180149-plasmid-map-sequence-id-357865

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  17. RelB cFlag pcDNA3

    Plasmid
    #20017
    Depositor
    Stephen Smale
    Insert
    RelB (Relb Mouse)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    20017-plasmid-map-sequence-id-197332

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  19. pMega-MaPylRS

    Plasmid
    #200225
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    none
    Promoter
    proK-lacO and tacI
    Available since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    200225-plasmid-map-sequence-id-400630

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  20. pSBtet-RP

    Plasmid
    #60497
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60497-plasmid-map-sequence-id-97550

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  21. AbVec_IGKC

    Plasmid
    #183702
    Purpose
    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.
    Depositor
    David Nemazee
    Insert
    IGKC (IGKC )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    183702-plasmid-map-sequence-id-358531

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  22. TFORF1690

    Plasmid
    #143967
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF1A (HNF1A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143967-plasmid-map-sequence-id-338769

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  23. p406GAL1

    Plasmid
    #17437
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17437-plasmid-map-sequence-id-8611

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  27. pmCherry-N1-GalT

    Plasmid
    #87327
    Purpose
    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87327-plasmid-map-sequence-id-177633

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  29. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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  32. TFORF1284

    Plasmid
    #143946
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SALL1 (SALL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143946-plasmid-map-sequence-id-404607

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  40. pSL1425 (pSPIN, pCDF backbone)

    Plasmid
    #160735
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    160735-plasmid-map-sequence-id-317231

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  41. FZ-NbPDS

    Plasmid
    #184268
    Purpose
    pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.
    Depositor
    Feng Qu
    Article
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP
    Use
    Tags
    Expression
    Plant
    Mutation
    The protease processing site between MP and L-CP …
    Promoter
    CaMV 35S with duplicated enhancer
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184268-plasmid-map-sequence-id-363057

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  42. QUIN

    Plasmid
    #184267
    Purpose
    A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.
    Depositor
    Feng Qu
    Article
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA1 cDNA with 4 introns
    Use
    Tags
    Expression
    Plant
    Mutation
    S1098P, K1651R
    Promoter
    CaMV 35S with duplicated enhancer
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184267-plasmid-map-sequence-id-363074

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  44. TFORF0695

    Plasmid
    #142677
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4A (HNF4A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142677-plasmid-map-sequence-id-397791

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  45. TFORF0691

    Plasmid
    #142214
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4A (HNF4A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142214-plasmid-map-sequence-id-397723

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  46. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  48. TFORF1472

    Plasmid
    #141825
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TOX2 (TOX2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141825-plasmid-map-sequence-id-415041

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Showing: 9551 - 9600 of 148090 results