-
Plasmid#41005DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-mutKir2.1
Plasmid#125579PurposeCre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationA non-conducting mutant (GYG -> AAA).PromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-mSHMT1
Plasmid#83904Purposestable overexpressionDepositorInsertSerine hydroxymethyltransferase 1 (Shmt1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR macroH2A1.2-C
Plasmid#30512DepositorInsertmacroH2A1.2-C (Macroh2a1 Mouse)
UseGateway - donor vectorTagsExpressionMutationNO STOPPromoterAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc mouse LNX2
Plasmid#37010DepositorInsertLNX2 (Lnx2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV566 A-USF
Plasmid#33360DepositorInsertUSF (Usf1 Mouse)
UseTagsHAExpressionMammalianMutationDominant Negative (see notes below)PromoterCMVAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Gria3-GFP KI
Plasmid#131491PurposeEndogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-2B-Tbx5
Plasmid#98619PurposeGateway entry vector TBX5DepositorInsertTBX5 (Tbx5 Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330 Ctnnb1.2
Plasmid#59912PurposepX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoterDepositorInsertCtnnb1 (Ctnnb1 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Drp1 V2 S579D
Plasmid#46343DepositorInsertDrp1 V2 (Dnm1l Mouse)
UseTagsExpressionMammalianMutationS579DPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pk-myc-Par6A
Plasmid#15472DepositorInsertPartitioning-defective protein 6A (Pard6g Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Hes1
Plasmid#36983DepositorInsertHes1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
(O75) FSP27/pLNCX2
Plasmid#25880DepositorInsertFSP27 (Cidec Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV eIF4E E103A IRES GFP
Plasmid#18766DepositorInserteIF4E E103A (Eif4e Mouse)
UseRetroviralTagsIRES GFPExpressionMammalianMutationE103APromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-mPRMT7-GFP
Plasmid#87104PurposeExpress mouse PRMT7 with Flag-tag in mammalian cellsDepositorInsertProtein arginine methyltransferase 7 (Prmt7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterEF1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2PromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mFgf21pro2-luc
Plasmid#101798PurposeReporter for mouse Fgf21 promoter activityDepositorInsertFibroblast growth factor 21 promoter (Fgf21 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE
Plasmid#111393PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)DepositorInsertsUseAAVTags6xHis, Myc, T7, and XpressExpressionMammalianMutationPromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ1A
Plasmid#197381PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-1 σ1A
UseTagsGAL4-DNA binding domain fragment, HA tag, and SV4…ExpressionYeastMutationContains an extra 42 nucleotides encoding 14 resi…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1
Plasmid#184398PurposeDonor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1DepositorInsertRenilla luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only