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Plasmid#152986PurposeGateway-compatible Entry vector, with insert of ORF10wu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020DepositorInsertORF10wu
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP13
Plasmid#154411PurposeGateway-compatible Entry vectorDepositorInsertNSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 2, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-RNAi-nucEGFPmiR-TEV-mCherry
Plasmid#105812PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-mCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-NSP2
Plasmid#154401PurposeGateway-compatible Entry vectorDepositorInsertNSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 ORF9Bwu_nostop
Plasmid#149328PurposeGateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020. Does not contain stop codon.DepositorInsertORF9Bwu
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-RNAi-nucEGFPmiR-mCherry-TEV
Plasmid#105808PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsmCherry-TEVExpressionMammalianMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-RNAi-nucEGFPmiR-FLAG-TEV
Plasmid#105807PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsFLAG-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 E 27nt-del
Plasmid#153954PurposeGateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYPQ289-RVR
Plasmid#138127PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoterDepositorInsertErCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD513R, K519V and N523RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVR
Plasmid#138117PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoterDepositorInsertMb2Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V and N573RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-NSP10
Plasmid#154409PurposeGateway-compatible Entry vectorDepositorInsertNSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-NSP7
Plasmid#154406PurposeGateway-compatible Entry vectorDepositorInsertNSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOEM1_pCMV:bmp4-pPH:vsvged
Plasmid#111157PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInserthBmp4 (BMP4 Human)
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-NSP9
Plasmid#154408PurposeGateway-compatible Entry vectorDepositorInsertNSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKK-RNAi-nucCHERRYmiR-TEV-FLAG
Plasmid#105813PurposeFunctional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.DepositorTypeEmpty backboneUseRNAi; Flp-in competentTagsTEV-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-ORF7B
Plasmid#154422PurposeGateway-compatible Entry vectorDepositorInsertORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-ORF6
Plasmid#154420PurposeGateway-compatible Entry vectorDepositorInsertORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 7, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-ORF7A
Plasmid#154421PurposeGateway-compatible Entry vectorDepositorInsertORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV2 TEV-ORF8
Plasmid#154423PurposeGateway-compatible Entry vectorDepositorInsertORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of o…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CHK2_HUMAN_D0
Plasmid#79715PurposeThis plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertCHK2 (CHEK2 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only