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Showing: 3781 - 3800 of 8972 results

  8. pCOA-Leu-FvPPT1

    Plasmid
    #179923
    Purpose
    Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Insert
    FvPPT1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179923-plasmid-map-sequence-id-359550

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  9. pGBT9-GGA2-GAE

    Plasmid
    #199175
    Purpose
    Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.
    Insert
    GGA2-GAE domain (GGA2 Human)
    Use
    Tags
    GAL4-DNA binding domain fragment
    Expression
    Yeast
    Mutation
    Contains Lys 545 Gln substitution
    Promoter
    ADH1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199175-plasmid-map-sequence-id-389860

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  14. pACT2-μ3A

    Plasmid
    #198174
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3A
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitution in codon 225
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198174-plasmid-map-sequence-id-387537

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  15. pACT2-μ1B (137-423)

    Plasmid
    #198172
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-1 μ1B (137-423)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198172-plasmid-map-sequence-id-387535

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  17. pROS13_IDH2 #7

    Plasmid
    #166102
    Purpose
    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166102-plasmid-map-sequence-id-330316

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  18. pROS13_IDH1 #4

    Plasmid
    #166101
    Purpose
    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166101-plasmid-map-sequence-id-330298

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Showing: 3781 - 3800 of 8972 results