Addgene

We narrowed to 15,711 results for: puromycin

Showing: 3781 - 3800 of 15711 results

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1Alpha and U6

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSuperRetropuro-IL-6 shRNA 1

Plasmid

#19959

Depositor

Christopher Counter

Article

Ancrile et al Genes Dev. 2007 Jul 15. 21(14):1714-9.

Insert

pSuperRetropuro-IL-6 shRNA 1 (IL6 Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
LentiCRISPRv2puro-Tyr

Plasmid

#170293

Purpose

A knock-out vector for the mouse tyrosinase.

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shFEN

Plasmid

#17921

Depositor

Sheila Stewart

Article

Saharia et al Curr Biol. 2008 Apr 8. 18(7):496-500.

Insert

shFEN (FEN1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
BII-ChPtW-PLAGL2

Plasmid

#133380

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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px459-RhebL1 sgRNA

Plasmid

#133769

Purpose

Expresses Cas9 and human RhebL1 sgRNA

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

RhebL1 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG NUFIP1

Plasmid

#110950

Purpose

expresses mammalian FLAG-NUFIP1

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-mPer2ER-pBABEpuro

Plasmid

#61430

Purpose

Express mPER2-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mPER2 (Per2 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Expression

Mutation

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pZR064_CROPseq-sgCD4-CRISPRi

Plasmid

#180270

Purpose

Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

CD4 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSR-puro-Mff1 shRNA

Plasmid

#37247

Depositor

David Kashatus, Christopher Counter

Article

Kashatus et al Nat Cell Biol. 2011 Aug 7;13(9):1108-15.

Insert

Mff1 (MFF Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pB-puro-V5-Trpt1

Plasmid

#126607

Purpose

Expresses N-terminal V5-tagged mouse Trpt1

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

tRNA 2' phosphotransferase 1 (Trpt1 Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK2

Plasmid

#38201

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

Unc-51 like kinase 2 (Ulk2 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pMSCVpuroATT

Plasmid

#47539

Purpose

Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

Depositor

David Weinstock

Article

Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only
pBabe-puro-mIR-31

Plasmid

#26088

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2010

Availability

Academic Institutions and Nonprofits only
pCDH-CRY2-mCh-superPLDx10

Plasmid

#188986

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Use

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSMP-SUZ12_1

Plasmid

#36390

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SUZ12 (SUZ12 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-LOV

Plasmid

#22033

Purpose

Mammalian expression of mVenus tagged LOV2 domain

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-LOV

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Insert length based on Venus (720bp) + LOV (450bp)

Promoter

Available since

Sept. 11, 2009

Availability

Academic Institutions and Nonprofits only
pQCXIP X2 DEST (w270-1)

Plasmid

#17474

Purpose

Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi and Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ-mgU2-47

Plasmid

#127585

Purpose

Expresses mgU2-47 scaRNA from a mU6 promoter

Depositor

David Rueda

Article

Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

Insert

mgU2-47 scaRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

modified U6

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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