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pLG010 (DRT type 2)

Plasmid

#157888

Purpose

Expresses the DRT type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TA-ERP2

Plasmid

#80477

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pHB-4

Plasmid

#140957

Purpose

For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

Depositor

Kang Zhou

Article

Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

Insert

phaCAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCA24N_6xHis-TEV-rpsA

Plasmid

#83585

Purpose

Express E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tag

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

rpsA (rpsA Escherichia coli)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

p-T5 Lac

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXP CMV/TO DEST (w360-1)

Plasmid

#17386

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLP1

Plasmid

#209988

Purpose

Entry vector to store Level 0 parts

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pMpGWB109

Plasmid

#68563

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLG007 (Ec86 retron)

Plasmid

#157885

Purpose

Expresses the Ec86 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec86 retron

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDSW1728

Plasmid

#120812

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

Depositor

David S. Weiss, Craig Ellermeier

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRU325

Plasmid

#167676

Purpose

Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBPGUw

Plasmid

#17575

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi

Plasmid

#53277

Purpose

Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

Insert

idi

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Promoter

endogenous promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xFLAG-V5-ccdB

Plasmid

#87064

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRU295

Plasmid

#167694

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Dest-pA-pgk-bsd

Plasmid

#74918

Purpose

Gateway destination piggyBac vector

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
pMpGWB327

Plasmid

#68655

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

TagRFP

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge