Addgene

We narrowed to 4,156 results for: 027

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LN-entry

Plasmid

#128012

Purpose

Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

LN-3xFlag

Use

Lamdan entry vector

Tags

LN-3xFlag

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(K271Q)

Plasmid

#133270

Purpose

X. laevis expression vector. It will generate the mouse TREK-1 channel

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )

Use

Oocyte expression

Tags

Expression

Mutation

K271Q

Promoter

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(Q258K)

Plasmid

#133077

Purpose

X. laevis expression vector. It will generate the human TRAAK channel

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)

Use

Tags

Expression

Mutation

Q258K

Promoter

T7

Available since

Oct. 30, 2021

Availability

Academic Institutions and Nonprofits only

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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Use

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1H01

Plasmid

#71090

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

nej (nej Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pHis6-hDF

Plasmid

#72585

Purpose

human dysferlin full length tagged with His

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin (DYSF Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
33-G-X1-pEF1-puro

Plasmid

#111604

Purpose

Express human 33-G-X1 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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33-G-X5-pEF1/V5-His

Plasmid

#111605

Purpose

Express human 33-G-X5 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Use

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRP_8xgRNA-FLuc

Plasmid

#135937

Purpose

Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

minCMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB)

Plasmid

#169721

Purpose

Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCINeoFlag ALIX; I318A

Plasmid

#89867

Purpose

Expresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

ALIX (PDCD6IP Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Mutated isoleucine 318 to alanine

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF C2B

Plasmid

#72583

Purpose

human dysferlin C2B domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2B domain (DYSF Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pEYFP-hDF-C2A

Plasmid

#72582

Purpose

human dysferlin C2A domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2A domain (DYSF Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

C2A domain

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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r-22. pT7-7-SNCA-9Asn

Plasmid

#107297

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-9Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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t2. pT7-7-SNCA-5Lys

Plasmid

#107294

Purpose

Plasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Lys (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r-6. pT7-7-SNCA-2Asn

Plasmid

#107296

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-2Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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r. pT7-7-SNCA-5Asn

Plasmid

#107295

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asn (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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s2. pT7-7-SNCA-5Asp

Plasmid

#107293

Purpose

Plasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 Gly

Depositor

Dmytro Yushchenko

Article

Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

Insert

SNCA-5Asp (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00379

Plasmid

#34494

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8208 (MBD-like Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only