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pCDNA3.1-PPARA

Plasmid

#169019

Purpose

Expression of PPARA driven by CMV promoter

Depositor

Claes Wadelius

Article

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human ppara (PPARA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA3m

Plasmid

#208702

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA3m

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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NYESO beta/alpha into TRAC HDRT Source (pTR 169)

Plasmid

#112021

Purpose

DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

Depositor

Alexander Marson

Article

Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

NYESO beta/alpha into TRAC HDRT

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1A-SynTetOff-GFP

Plasmid

#190230

Purpose

Gateway entry vector, high-level expression of EGFP in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNeo2/Cre

Plasmid

#89652

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro

Plasmid

#89992

Purpose

Donor template for generation of OCT4-T2A-nEmGFP reporter cell lines

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

OCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBITE-CD19-CD3

Plasmid

#190677

Purpose

Encodes a modularized form of HD37-OKT3 bispecific antibody

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Modularized-HD37-modularized-OKT3-P2A-NeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-Pol-II WT

Plasmid

#35175

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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PyronicSF/pcDNA3.1(-)

Plasmid

#124812

Purpose

Highly resposive GFP-based pyruvate nanosensor.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al Elife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSNAP-tag (m) Vector

Plasmid

#101135

Purpose

Expression of protein of interest with SNAP-Tag in Mammalian cells

Depositor

Ana Egana, New England Biolabs

Type

Empty backbone

Use

Tags

SNAP-tag (SNAP26m)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CN3098-pAAV-eHGT_380h-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208123

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cre-P2A-dTomato

Plasmid

#107738

Purpose

Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

Depositor

Rylan Larsen

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Cre, dTomato

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-NLS-HA-TurboID

Plasmid

#215075

Purpose

Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-EB1-EGFP

Plasmid

#118084

Purpose

Expresses EB1-EGFP in mammalian cells

Depositor

Ken-Ichi Takemaru

Insert

human EB1 (MAPRE1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

EF1 alpha

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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LentiCROP-PuroR-P2A-GFP-U6

Plasmid

#200029

Purpose

Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

Depositor

Jay Shendure

Type

Empty backbone

Use

Lentiviral

Tags

PuroR-P2A-GFP

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CN4253-rAAV-3xCore2_eHGT_380h-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#208165

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus

Depositor

Jonathan Ting, The Allen Institute for Brain Science

Article

Mich et al bioRxiv 2023.09.20.558718

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Beta Globin minimal promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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