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Plasmid#10865DepositorInsertbetaTrCP (BTRC Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
ELONGIN BC (XX01TCEB1A-c001)
Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorUseTagsExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7Available sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAPDH-CPG-TV
Plasmid#70148Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyADepositorInsert5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
UseMouse Targeting and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
VP12
Plasmid#72247PurposeHuman expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A and Q926APromoterCMVAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
RedTrackCMV
Plasmid#50957PurposeA backbone for adenovirus creation by the AdEasy method, similar to AdTrackCMV (Addgene Plasmid # 16405), except it co-expressed mRFP instead of eGFPDepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKM201
Plasmid#13078DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pCDNA3
Plasmid#107173Purposeexpresses V5-tagged TurboID on the mammalian ER membraneDepositorInsertTurboID (BirA mutant)
UseTagsExpressionMammalianMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-VEGFR2-N
Plasmid#54298PurposeLocalization: Membrane Receptor, Excitation: 487, Emission: 509DepositorInsertVEGFR2 (KDR Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Emerin pEGFP-C1 (637)
Plasmid#61993Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertEmerin (EMD Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Ascl1
Plasmid#27150DepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianMutationPromoterCBhAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV3flag8HOIL-1L
Plasmid#50016PurposeMammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertRanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLOX-TERT-iresTK
Plasmid#12245Purpose2nd generation lentiviral vector for reversible immortalization of human primary cellsDepositorInsertTERT, HSV1-TK (TERT Human)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-His7tag-Peredox-mCherry
Plasmid#32382DepositorInsertHis7-Peredox-mCherry
UseTagsHis7tagExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-H2B-6
Plasmid#55243PurposeLocalization: Nucleus/Histones, Excitation: 383, Emission: 448DepositorInsertH2B (H2BC11 Human)
UseTagsEBFP2ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-Beclin 1
Plasmid#86748PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman Beclin 1 (BECN1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-FLNa WT
Plasmid#8982DepositorInsertFilamin A (FLNA Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
pmTurquoise2-H2A
Plasmid#36207PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only